Hydrolysis of glucosinolates using nylon-immobilized myrosinase to produce pure bioactive molecules
2000; Wiley; Volume: 68; Issue: 6 Linguagem: Inglês
10.1002/(sici)1097-0290(20000620)68
ISSN1097-0290
AutoresOnofrio Leoni, Renato Iori, Sandro Palmieri,
Tópico(s)Coffee research and impacts
ResumoBioactive compounds were produced from natural glucosinolates, secondary plant metabolites, using myrosinase (thioglucoside glucohydrolase EC 3.2.3.1) isolated from ripe seeds of Sinapis alba. The enzyme was immobilized on granular nylon 6.6 with the crosslinking technique. Immobilized myrosinase displayed extraordinary operational and storage stability. Using a small thermostatted continuous packed-bed bioreactor, the enzyme activity was unchanged after 15 days of continuous use at 37°C and after >1 year of storage at room temperature. The bioreactor was particularly efficient in producing pure isothiocyanates, but it was less efficient for pure nitrile production. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 660–664, 2000.
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