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BIBTEX RIS

Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans : Radiation Sensitivities of Catalase ( katA ) and Superoxide Dismutase ( sodA ) Mutants

1999; American Society for Microbiology; Volume: 181; Issue: 2 Linguagem: Inglês

10.1128/jb.181.2.666-669.1999

ISSN

1098-5530

Autores

Lye Meng Markillie, Susan M. Varnum, Preston Hradecky, Kwong‐Kwok Wong,

Tópico(s)

Microbial bioremediation and biosurfactants

Resumo

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

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