Persistence Length of Single-Stranded DNA
1997; American Chemical Society; Volume: 30; Issue: 19 Linguagem: Inglês
10.1021/ma970381+
ISSN1520-5835
AutoresB. Tinland, Alain Pluen, Jean Sturm, G. Weill,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoThe self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. The total persistence length p of single-stranded DNAs and its variation in ionic strength down to 10-3 M has been deduced. The importance of the value of p versus the pore size a and contour length L of the DNA in the optimization of sequencing by gel electrophoresis is emphasized.
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