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The MetaProteomeAnalyzer: A Powerful Open-Source Software Suite for Metaproteomics Data Analysis and Interpretation

2015; American Chemical Society; Volume: 14; Issue: 3 Linguagem: Inglês

10.1021/pr501246w

1535-3907

Thilo Muth, Alexander Behne, Robert Heyer, Fabian Kohrs, Dirk Benndorf, Marcus Hoffmann, Miro Lehtevä, Udo Reichl, Lennart Martens, Erdmann Rapp,

Microbial Community Ecology and Physiology

The enormous challenges of mass spectrometry-based metaproteomics are primarily related to the analysis and interpretation of the acquired data. This includes reliable identification of mass spectra and the meaningful integration of taxonomic and functional meta-information from samples containing hundreds of unknown species. To ease these difficulties, we developed a dedicated software suite, the MetaProteomeAnalyzer, an intuitive open-source tool for metaproteomics data analysis and interpretation, which includes multiple search engines and the feature to decrease data redundancy by grouping protein hits to so-called meta-proteins. We also designed a graph database back-end for the MetaProteomeAnalyzer to allow seamless analysis of results. The functionality of the MetaProteomeAnalyzer is demonstrated using a sample of a microbial community taken from a biogas plant.