Portal de Periódicos da CAPES

Contrasting globulin and cysteine proteinase gene expression patterns reveal fundamental developmental differences between zygotic and somatic embryos of oil palm

2008; Oxford University Press; Volume: 28; Issue: 8 Linguagem: Inglês

10.1093/treephys/28.8.1157

1758-4469

Frédérique Aberlenc‐Bertossi, Nathalie Chabrillange, Yves Duval, James Tregear,

Cassava research and cyanide

Oil palm (Elaeis guineensis Jacq.) somatic embryos differ from zygotic embryos in that they accumulate only small amounts of storage proteins. We compared the balance between deposition and degradation of storage proteins during zygotic or somatic embryogenesis and germinative growth in the two types of embryos. During mid to late zygotic embryogenesis, storage proteins accumulated and globulin 7S (GLO7A) gene transcripts were detected, whereas neither protease activity nor cysteine proteinase (CPR) gene transcripts were detected. Globulin degradation occurred after 8 days of in vitro germination in zygotic embryos and was accompanied by a decrease in GLO7A transcripts. Transcripts of three cysteine proteinase genes of the papain family were detected as early as Day 2 of in vitro germination. Several proteolytically active protein bands were identified by zymography, and CPR-like proteins were detected with an antibody raised against the Vicia sativa L. cysteine proteinase CPR1. Protease activities and CPR-like proteins were observed from Day 8 onward when globulin degradation occurred.