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Acetylation regulates the differentiation-specific functions of the retinoblastoma protein

2004; Springer Nature; Volume: 23; Issue: 7 Linguagem: Inglês

10.1038/sj.emboj.7600176

1460-2075

Don X. Nguyen, Laurel A. Baglia, Shih-Min A. Huang, Christina M. Baker, Dennis J. McCance,

Ubiquitin and proteasome pathways

Article25 March 2004free access Acetylation regulates the differentiation-specific functions of the retinoblastoma protein Don X Nguyen Don X Nguyen Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Laurel A Baglia Laurel A Baglia Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Shih-Min Huang Shih-Min Huang Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USAPresent address: Genomic Institute of the Novartis Foundation, San Diego, CA 9212, USA Search for more papers by this author Christina M Baker Christina M Baker Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Dennis J McCance Corresponding Author Dennis J McCance Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA The Cancer Center, University of Rochester, Rochester, NY, USA Search for more papers by this author Don X Nguyen Don X Nguyen Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Laurel A Baglia Laurel A Baglia Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Shih-Min Huang Shih-Min Huang Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USAPresent address: Genomic Institute of the Novartis Foundation, San Diego, CA 9212, USA Search for more papers by this author Christina M Baker Christina M Baker Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA Search for more papers by this author Dennis J McCance Corresponding Author Dennis J McCance Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA The Cancer Center, University of Rochester, Rochester, NY, USA Search for more papers by this author Author Information Don X Nguyen1, Laurel A Baglia1, Shih-Min Huang1, Christina M Baker1 and Dennis J McCance 1,2 1Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA 2The Cancer Center, University of Rochester, Rochester, NY, USA *Corresponding author. School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Box 672, Rochester, NY 14642, USA. Tel.: +1 585 275 0101; Fax: +1 585 473 9573; E-mail: [email protected], [email protected] The EMBO Journal (2004)23:1609-1618https://doi.org/10.1038/sj.emboj.7600176 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The retinoblastoma tumor-suppressor protein (pRb) is known to induce growth arrest and cellular differentiation. The molecular determinants of pRb function include protein–protein interactions and post-translational modifications such as phosphorylation. Recently, the co-activator p300 was found to acetylate pRb. The biological significance of pRb acetylation, however, remains unclear. In the present study, we provide evidence that pRb undergoes acetylation upon cellular differentiation, including skeletal myogenesis. In addition to p300, the p300-Associated Factor (P/CAF) can mediate pRb acetylation as pRb interacts directly with the acetyltransferase domain of P/CAF in vitro and can associate with P/CAF in differentiated cells. Significantly, by using a C terminal acetylation-impaired mutant of pRb, we reveal that acetylation does not affect pRb-dependent growth arrest or the repression of E2F transcriptional activity. Instead, acetylation is required for pRb-mediated terminal cell cycle exit and the induction of late myogenic gene expression. Based on these results, we propose that acetylation regulates the differentiation-specific function(s) of pRb. Introduction The retinoblastoma (Rb) gene family consists of three members, Rb, p107 and p130, known to encode 'pocket' proteins that regulate the mammalian cell cycle. More specifically, pRb family members have overlapping roles in promoting cellular growth arrest. Targeted inactivation of all the three Rb-related genes in embryonic stem cells causes deregulated G1/S transition, abrogation of G1 arrest and cellular immortalization (Dannenberg et al, 2000; Sage et al, 2000). The underlying mechanism of these observations involves the common ability of pocket proteins to interact with the E2F family of transcription factors. This is exemplified by the activity of pRb, which, once in a hypophosphorylated state, can bind to E2F-1 or E2F-3 and repress E2F-induced expression of genes normally required for cell cycle progression (Dyson, 1998; Harbour and Dean, 2000). Despite the apparent similarities, multiple studies have now revealed the biochemical and biological differences that distinguish pRb from p107 and p130 (Classon and Dyson, 2001). Most noteworthy is the fact that pRb is the only pocket protein known to exhibit features of a bona-fide tumor suppressor. Accordingly, Rb heterozygous mice are predisposed to the onset of pituitary and thyroid cancer (Clarke et al, 1992; Jacks et al, 1992), and deregulation of the Rb signaling pathway or inactivation of Rb itself is a hallmark of nearly all human tumors (Hanahan and Weinberg, 2000). Furthermore, genetic experiments have demonstrated a unique role for Rb in metazoan development and cellular differentiation. Germline deletion of Rb in mice, for instance, results in embryonic lethality between 13 and 15 days of gestation (Clarke et al, 1992; Jacks et al, 1992; Lee et al, 1992), whereas p107−/− or p130−/− mice are seemingly viable (Cobrinik et al, 1996; Lee et al, 1996). More recent reports, however, suggest that the penetrance of p107/p130 null phenotypes may depend on the genetic background (LeCouter et al, 1998a, 1998b). Nonetheless, Rb−/− animals exhibit distinct developmental abnormalities, with deregulated proliferation, cell death and defects in differentiation seen in multiple embryonic tissues. Although a compound E2F-1 deletion extends the survival of Rb−/− animals, these mice eventually die of disruptions in erythroid, pulmonary and muscular development (Yamasaki et al, 1998). Consequently, important function(s) of the pRb tumor suppressor are not restricted to the regulation of E2F activity during cell cycle progression. In vitro tissue culture models also support the notion that pRb possesses a unique role in mediating differentiation. This is best demonstrated by the requirement for Rb during skeletal myogenesis (Gu et al, 1993; Schneider et al, 1994; Zacksenhaus et al, 1996). Three coordinated yet distinct biological events are known to occur during muscle differentiation. The first involves an initial growth arrest linked to the upregulation of the cell cycle inhibitor p21cip1 and repression of E2F-regulated proliferative genes. This early phase of the myogenic program does not seem to necessitate pRb, as other pocket proteins can substitute for loss of Rb in inducing growth arrest and Rb−/− myoblasts are capable of expressing early markers of differentiation (Schneider et al, 1994; Novitch et al, 1996). Following this acute growth arrest, a more permanent cell cycle withdrawal is considered necessary for the later stages of differentiation. This then renders myotubes conducive to the expression of late markers including the myosin heavy chain (MyHC). Rb-deficient cells are incapable of undergoing terminal arrest, exhibit delayed expression of late differentiation markers and can re-enter the cell cycle. Significantly, the aberrant myogenic phenotype associated with Rb disruption does not occur in cells lacking p107 or p130 (Novitch et al, 1996). Thus, pRb fills a more specific role throughout the later phases of myogenesis. Consistent with animal models, the ability of pRb to promote differentiation is independent of acute G1/S block and does not require stable binding to E2F (Sellers et al, 1998). Instead, preliminary efforts led to the idea that pRb can potentiate the muscle-specific transcription factor MyoD (Gu et al, 1993). Although several studies have confirmed the ability of pRb to stimulate MyoD-dependent transcription, this cooperation is probably of an indirect nature, as the association of pRb with MyoD in vivo remains controversial (Li et al, 2000). Alternative models propose that pRb binding to inhibitors of MyoD, such as the E1A-like inhibitor of differentiation-1 (EID-1) or histone deacetylases (HDACs), leads to 'de-repression' of MyoD activity (MacLellan et al, 2000; Miyake et al, 2000; Puri et al, 2001). Multiple biochemical factors are likely to contribute to the overall functions of pRb required for differentiation. In addition to pRb, other proteins are known to cooperate with MyoD in promoting muscle differentiation. Among these are the transcriptional co-activators CREB-binding protein (CBP), p300 and the p300-associated factor (P/CAF), which possess intrinsic acetyltransferase activity (Bannister and Kouzarides, 1996; Ogryzko et al, 1996; Yang et al, 1996) and are required for myogenesis (Puri et al, 1997; Polesskaya et al, 2001b). p300 and CBP are in fact known to regulate the activity of numerous transcription factors (Vo and Goodman, 2001), and possess conserved functions in cell growth, transformation and development (Goodman and Smolik, 2000). P/CAF has likewise been linked to differentiation and tumorigenesis (Yang et al, 1996; Schiltz and Nakatani, 2000). Current models propose that p300/CBP and P/CAF can form a multimeric complex with MyoD, which recruits these co-activators to muscle-specific promoters (Eckner et al, 1996; Yuan et al, 1996; Puri et al, 1997; Sartorelli et al, 1997). P/CAF and p300/CBP are then believed to hyperacetylate the surrounding nucleosomes, thus increasing the accessibility of additional transcription factors to MyoD target promoters (McKinsey et al, 2001). Interestingly, besides acetylating histone tails, p300/CBP and P/CAF can acetylate MyoD itself at conserved lysine residues (Sartorelli et al, 1999; Polesskaya et al, 2000; Polesskaya and Harel-Bellan, 2001). The acetylation of MyoD is postulated to increase DNA binding and is important for MyoD activity. Remarkably, p300/CBP and P/CAF have now been demonstrated to acetylate a wide variety of non-histone proteins, including many DNA-binding proteins, also general transcription factors, cytoplasmic proteins and tumor suppressors (Kouzarides, 2000). Recently, the co-activator p300 was shown to acetylate pRb in vitro (Chan et al, 2001). The mechanism and biological relevance of pRb acetylation, however, remain unclear. In the present study, we provide evidence that the differentiation-specific functions of pRb are modulated by acetylation. Results pRb is acetylated upon cellular differentiation The aim of the present study was to determine the importance of pRb acetylation during cellular differentiation. To this end, the acetylation state of pRb was examined in various differentiation cell culture systems. Murine C2C12 myoblasts are induced to differentiate into myotubes by growth to confluence and depletion of serum (DM). Using this system, expression of the late myogenic marker, MyHC, was detected by 36 h (Figure 1A, left). Acetylation of pRb was examined via immunoprecipitation of C2C12 nuclear extracts using either anti-pRb or control IgG antibody, followed by immunoblotting with an antibody that detects acetylated lysine residues (Ac-K). As shown in Figure 1A (right panel), acetylated pRb was more prevalent in differentiated (DM) extracts when compared to undifferentiated cells (GM). When the same blot was stripped and re-probed for pRb, a predominance of the active, hypophosphorylated (faster migrating) form of pRb was detected in differentiated myotubes. The acetylation state of pRb was also assessed in human foreskin keratinocytes (HFKs) that undergo cellular differentiation upon suspension in methylcellulose. Significantly, pRb became acetylated as the HFK differentiation marker involucrin was induced (Figure 1B). Taken together, these results demonstrate that pRb acetylation occurs upon cellular differentiation and that this regulatory process is conserved. Figure 1.pRb is acetylated upon cellular differentiation. (A) Asynchronous C2C12 cells were grown in 20% FCS and harvested at low confluence (GM), or grown to high confluence and incubated in 1% horse serum (DM). Samples were harvested following 18 and 36 h in DM. C2C12 differentiation was confirmed by immunoblotting for MyHC from whole-cell lysates (left). In the right panel, acetylation of pRb was detected by immunoprecipitating nuclear-enriched extracts with IgG control or an anti-pRb agarose-conjugated antibody. Immunoprecipitated samples were run on an SDS–PAGE gel, transferred to nitrocellulose and probed with anti-acetylated lysine (Ac-K) antibody. Blots were stripped and re-probed using anti-pRb to confirm the presence of an overlapping pRb band. (B) Asynchronous primary HFKs were either harvested as cycling (cyc) or suspended in methylcellulose. Differentiated cells (diff) were collected after 24 h in methylcellulose, and differentiation-induced expression of involucrin was confirmed by RT–PCR (left panel). The acetylation state of pRb was detected as in (A). Download figure Download PowerPoint Myogenesis induces pRb to associate with P/CAF As P/CAF is necessary for both muscle and keratinocyte differentiation (Puri et al, 1997; Kawabata et al, 2002), we examined whether pRb can form a complex with P/CAF. As demonstrated in Figure 2B, in vitro translated (IVT) P/CAF bound to the pRb large pocket (pRbLp). Deletion mutations at exons 21 and 22 (pRbLpΔ21, pRbLpΔ22) in the pocket region did not noticeably affect binding to P/CAF. The pRbA (aa 379–610, Figure 2A) domain alone did not pull down P/CAF, while the B (aa 612–767) and C (aa 792–928) regions of pRb interacted with P/CAF, albeit less efficiently when used individually. To confirm that pRb binds directly to P/CAF, the His-tagged HAT domain of P/CAF (aa 353–658, Figure 2A) was purified from bacteria and used in pull-down assays with full-length recombinant pRb (GST-pRbFl). This HAT domain was sufficient to interact directly with pRb (Figure 2C), and was as potent as the association of P/CAF with p53, a well-known in vitro and in vivo binding partner of P/CAF (Liu et al, 1999). Next, we used a recombinant P/CAF fragment lacking its first 351 amino acids (ΔN-P/CAF) in an attempt to co-purify endogenous pRb from differentiated C2C12 extract. ΔN-P/CAF does not interact with p300 (Reid et al, 1998), yet readily precipitated endogenous pRb (Figure 2D). Thus, P/CAF and pRb might form a complex in cells independently of p300. Having established that pRb and P/CAF interact in vitro, we attempted to determine whether such a complex could be detected in vivo. As pRb levels increase upon myogenesis (Figure 1A), GM and DM nuclear lysates were standardized for equal pRb levels before being immunoprecipitated. Consequently, pRb was found to co-precipitate with endogenous P/CAF and this association was significantly increased in differentiated cells (Figure 2E). Furthermore, endogenous P/CAF binding correlated with the hypophosphorylated and acetylated form of pRb (Figure 2E, lower panels). Similar observations were obtained in differentiating HFKs (data not shown). Thus, cellular differentiation induces P/CAF to associate with pRb. Figure 2.pRb associates with P/CAF. (A) Schematic diagram of pRb and P/CAF fragments used in this study. (B) In vitro translated 35S-labeled full-length P/CAF was incubated with the indicated GST-pRb fragments in a standard pull-down assay. Samples were resolved via SDS–PAGE gel; bound P/CAF was analyzed using phosphoimaging, while appropriate GST loading was confirmed via coomassie staining of a replica gel (bottom). Dots designate the running positions of GST proteins used. (C) Full-length GST-pRb or GST-p53 were incubated with the His-tagged HAT domain of P/CAF in a pull-down experiment and analyzed via Western blot. (D) GST-ΔN-P/CAF (Δ1–351) was used to purify endogenous pRb from differentiated C2C12 extracts. (E) GM or DM C2C12 extracts were standardized for pRb levels, immunoprecipitated as in Figure 1 and subjected to Western blot analysis for endogenous P/CAF. Samples immunoprecipitated in parallel were blotted with anti-Ac-K. The Ac-K blot was stripped and re-probed with anti-pRb. Download figure Download PowerPoint P/CAF mediates acetylation of pRb To assess whether P/CAF could also directly catalyze the acetylation of pRb, we used GST-pRbLp as a putative substrate for the HAT domain of P/CAF in an acetyltransferase assay. In addition, acetylation of pRbLp was compared to that of the small pocket (pRbSp: aa 379–792), which lacks the C terminus of pRb. Recombinant P/CAF was able to acetylate specifically the large pocket of pRb up to five-fold over background levels, as detected via Western blot (Figure 3A). Conversely, the small pocket of pRb was not markedly acetylated, even though it was capable of binding to P/CAF. We extrapolate from this result that the C terminus of pRb contains a major site(s) targeted by P/CAF for acetylation. Importantly, P/CAF could also enhance pRb acetylation in differentiating C2C12 cells (Figure 3B, lane 3). When an inactive version of P/CAF lacking its acetyltransferase domain (Flag-P/CAFΔHAT) was co-transfected along with HA-pRb, pRb acetylation was reduced, potentially due to a lower capacity of P/CAFΔHAT to associate with pRb (Figure 3B, lane 4). Consistent with a role for p300 in pRb acetylation, overexpression of p300 in C2C12 cells also increased detectable levels of acetylated pRb (Figure 3B, lane 5). Interestingly, co-transfection of P/CAF and p300 along with pRb resulted in an overall increase in pRb acetylation compared to samples transduced with either co-activator alone (Figure 3B, last lane). In sum, we conclude that P/CAF is a mediator of pRb acetylation, and that it can cooperate with p300 in fulfilling this activity. Figure 3.P/CAF mediates acetylation of pRb. (A) GST-pRbLp or GST-pRbSp were incubated with His-P/CAF(HAT) in an acetyltransferase assay with cold acetyl-CoA. Reaction products were separated via SDS–PAGE. Upper panel: Ac-K Western blot. Lower panel: coomassie-stained replica gel. (B) C2C12 cells were transfected with the indicated constructs before incubation in DM for 24 h. Lysates were immunoprecipitated with anti-HA(pRb) and subjected to Western blot analysis for the presence of acetylated pRb using anti-Ac-K. Dots indicate the presence of Flag-P/CAF or Flag-P/CAFΔHAT. (C) Schematic diagram of the pRb.RR mutant, in which lysines 873/874 in the C terminus have been mutated to non-acetylatable arginine residues. (D) GST-pRbLp or GST-pRbLp.RR were incubated with His-P/CAF(HAT) and radiolabeled 14C-acetyl-CoA as previously mentioned. (E) COS-1 cells were co-transfected with Flag-P/CAF and either wild-type pRb or the pRb.RR mutant. COS-1 cells were used here as they do not undergo differentiation and provide more limiting conditions for acetylation in cells. Acetylation of pRb or pRb.RR was determined as in (B). (F) Cells were transfected as in (E). Lysates were immunoprecipitated with anti-Flag (P/CAF) and blotted for HA (pRb/pRb.RR). Download figure Download PowerPoint Previously, p300 was found to acetylate the C terminus of pRb in vitro at lysine residues 873/874 (Chan et al, 2001). As our results demonstrate that P/CAF equally acetylates the C terminus of pRb in vitro, and that P/CAF cooperates with p300 in acetylating pRb in cells, we reasoned that amino acids 873/874 of pRb might be targeted by P/CAF as well. To verify this hypothesis, lysine residues 873/874 of pRb were mutated to arginine (RR), a change that preserves the overall charge, but inhibits acetylation of the sites in question. The resulting mutant is referred to as pRb.RR (Figure 3C). When used in vitro within the context of pRbLP, the RR mutant displayed a reduced capacity for P/CAF-mediated acetylation (Figure 3D). This was confirmed in COS-1 cells that were co-transfected with Flag-P/CAF and full-length pRb or pRb.RR (Figure 3E). Moreover, this decrease in acetylation was not caused by defects in P/CAF binding, as pRb.RR co-precipitates with Flag-P/CAF (Figure 3F). We note that the residual acetylation of pRb.RR is likely due to the presence of additional unidentified acetylated sites. Nevertheless, we conclude that lysine residues 873/874 are important sites of pRb acetylation. pRb acetylation is required for permanent cell cycle withdrawal and differentiation-specific gene expression To identify which biological function(s) requires pRb acetylation, the acetylation-impaired mutant pRb.RR was expressed in various Rb-deficient cell lines. Consistent with previous experiments, the expression of either wild-type pRb or pRb.RR in Rb−/− SAOS-2 cells resulted in G1/S growth arrest (data not shown) (Chan et al, 2001), demonstrating that pRb acetylation is not required for acute cell cycle arrest. Following these results, the requirement for pRb acetylation was more specifically examined within the context of cellular differentiation. As the genetic requirement for Rb during muscle development has been firmly established, skeletal myogenesis was used as our model system. Moreover, Rb−/− 3T3 fibroblasts were employed because ectopic expression of MyoD in normal 3T3 cells can induce myogenesis, while MyoD-Rb−/− cells do not differentiate properly and are incapable of undergoing permanent cell cycle withdrawal (Novitch et al, 1996). 3T3 cells were infected with amphotrophic retrovirus encoding MyoD or an empty vector to create stable cell lines (MyoD-Rb−/− or pBabe-Rb−/−) that were then transfected with pRb or the pRb.RR mutant. Finally, subconfluent cells were either starved in DM media for 24 h (DM) or starved for 48 h before being re-stimulated with 10% serum for 18 h (serum). When MyoD was stably expressed alone in an Rb−/− background, cells failed to undergo growth arrest upon incubation in DM, with approximately 15% of GFP-positive cells maintaining DNA synthesis as measured by BrdU incorporation (Figure 4A). This is in agreement with the fact that Rb−/− 3T3 fibroblasts are deficient for growth arrest upon serum starvation (Classon et al, 2000) and that MyoD-induced arrest requires Rb (Peschiaroli et al, 2002). Predictably, these samples had a higher proliferative potential when re-incubated in serum-containing media. Expression of pRb in MyoD-Rb−/− fibroblasts rescued the arrest mediated by growth factor depletion and further rendered transduced cells refractory to mitogenic stimulation (Figure 4A), the latter being a hallmark of cells committed towards differentiation. When pRb.RR was introduced into a MyoD-Rb−/− background, cells initially arrested indistinguishably from wild-type pRb when starved. Importantly though, 15% of pRb.RR transfected cells re-entered the cell cycle upon addition of serum, indicating that cells expressing the acetylation defective mutant maintain their proliferative potential. Similar experiments were performed with CC42 muscle precursor cells, which do not exhibit terminal growth arrest upon differentiation due to impaired pRb function (Schneider et al, 1994; Chen and Wang, 2000). Stable expression of pRb or pRb.RR in CC42 myotubes confirmed that the acetylation defective mutant could not establish a growth refractory state (Figure 4B). Moreover, while wild-type pRb retained a more hypophosphorylated (p) state following serum re-stimulation, pRb.RR was predominantly hyperphosphorylated (pp), consistent with its inability (Figure 4C) to establish permanent cell cycle withdrawal. Figure 4.Acetylation of pRb at lysine residues 873/874 is required for permanent cell cycle withdrawal. (A) Rb−/− fibroblasts were infected with amphotrophic retrovirus expressing MyoD or empty vector. Following selection, stable control Rb−/− or MyoD-Rb−/− cell lines were transfected with pRb.wt, pRb.RR or vector control. A GFP construct was included in all samples at 1/10 the total amount of DNA. Cells were then either starved in DM for 24 h or starved for 48 h before being re-stimulated with 10% serum, labeled with BrdU and fixed for immunostaining. Proliferative cells incorporating BrdU were identified under fluorescent microscopy and scored as a percentage of BrdU-GFP double-positive cells over the total number of GFP transfected cells. (B) CC42 myoblasts were co-transfected with pRb or pRb.RR along with a puromycin-expressing vector. Stable transformants were isolated following selection, incubated in GM (asynchronous cycling), DM (for 72 h) or DM followed by re-stimulation in 20% serum for 18 h. BrdU-incorporating cells were analyzed via flow cytometric analysis. (C) Immunoblots detecting HA-pRb from stable CC42 lines in (B). Download figure Download PowerPoint As acetylation of pRb is necessary to cooperate with MyoD in establishing permanent growth arrest, we hypothesized that pRb acetylation might also affect other MyoD-dependent functions, such as differentiation-specific gene expression. To test this, MyoD-Rb−/− lines were transfected as in Figure 4 and the steady-state levels of myogenic markers were examined. As previously demonstrated, pRb had little or no effect on MyoD-dependent expression of the early markers of differentiation myogenin (Figure 5A, left) and p21cip1 (right), while both MyoD and pRb were required for optimal induction of the late marker MyHC (Figure 5A). When pRb.RR was expressed, no defects were seen on myogenin or p21cip1 expression. However, induction of MyHC was reduced compared to wild-type pRb/MyoD-expressing cells, suggesting a failure of the pRb.RR mutant to stimulate MyoD activity fully. Finally, immunofluorescent microscopy revealed that even at later time points (DM=72 h), cells expressing pRb.RR displayed a more diffuse pattern of MyHC expression (Figure 5B) as well as an overall lower percentage of MyHC-positive cells compared to wild-type pRb (Figure 5C). We conclude from these results that acetylation of pRb at residues 873/874 is dispensable for the early stages of differentiation, including acute cell cycle arrest and expression of early markers. More importantly, pRb acetylation is required to establish permanent cell cycle withdrawal and induction of late differentiation gene expression. Figure 5.Acetylation of pRb at lysine residues 873/874 is required for optimal expression of late differentiation markers. (A) Control Rb−/− or MyoD-Rb−/− cells were transfected as in Figure 4, grown to confluence and starved in DM for the indicated times. Steady-state levels of MyHC, myogenin, p21cip1, MyoD and pRb were assessed via Western blot. MyoD appears as a doublet, previously described as different phosphorylated forms. Endogenous MyoD was detectable upon overexposure. Nonspecific (NS) bands serve as loading controls. (B) MyoD-Rb−/− samples were transfected with pRb or pRb.RR along with GFP and fixed after 72 h in DM for MyHC and myogenin immunostaining. Depicted are representative fields (similar exposure times) of GFP and MyHC-positive cells. (C) Quantitation of MyHC/GFP and myogenin/GFP-positive cells following 72 h in DM. Download figure Download PowerPoint Mutation of pRb-acetylated residues 873/874 does not affect E2F transcriptional repression, but results in impaired MyoD-dependent transactivation The molecular properties of pRb can be categorized into two distinct activities: (1) pRb transcriptional repression of cell cycle-regulated genes and (2) its ability to act as a differentiation-specific co-activator. To determine whether these activities are regulated by acetylation, the pRb.RR mutant was used in transcriptional reporter assays. In the first set of experiments, the ability of pRb.RR to repress E2F-1 transcription was examined. Rb−/− 3T3 cells were transfected with Gal4-E2F-1, a Gal4 reporter plasmid and pRb or the indicated mutants. Luciferase activity was measured following 24 h of serum starvation. The pocket mutant pRbΔ22, which does not induce cell cycle arrest or muscle-specific gene expression (Sellers et al, 1998), was included as a negative control and is unable to repress E2F transcription when compared to wild-type pRb (Figure 6A). In contrast, pRb.RR was still capable of significantly repressing E2F activity. Furthermore, co-transfection of P/CAF with wild-type pRb did not alter E2F repression (data not shown). In Figure 6B, pRb-mediated co-activation was analyzed using a reporter construct responsive to MyoD activation. Rb-negative cell lines were transfected with 4RE-luc, MyoD and limiting amounts of pRb or the indicated mutants, followed by incubation in DM. Interestingly, while pRb was found to stimulate MyoD activity, pRb.RR was impaired in co-activating MyoD-dependent transcription (Figure 6B). These results were confirmed by examining the endogenous levels of cyclin A, a marker of proliferation that is repressed by pRb, and MyHC that is activated by MyoD (Figure 6C). Acetylation is thus required for pRb to function as a co-activator, but dispensable for transcriptional repression. Figure 6.Mutation of pRb-acetylated residues 873/874 does not affect E2F transcriptional repression, but results in impaired MyoD-dependent transactivation. (A) Rb−/− 3T3 cells were transfected with plasmids encoding the E2F-1 transactivating domain fused to Gal-4 (Gal4-E2F), pRb or the indicated pRb mutants, and a Gal-4 luc reporter. Following transfection, cells were st