Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System
2002; American Society for Microbiology; Volume: 40; Issue: 7 Linguagem: Inglês
10.1128/jcm.40.7.2659-2661.2002
ISSN1098-660X
AutoresNabin K. Shrestha, Marion J. Tuohy, Gerri S. Hall, Carlos M. Isada, Gary W. Procop,
Tópico(s)Infective Endocarditis Diagnosis and Management
ResumoABSTRACT One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.
Referência(s)