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ALTERATIONS IN GAP JUNCTION PROTEIN EXPRESSION IN HUMAN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCER

2002; Lippincott Williams & Wilkins; Volume: 167; Issue: 2 Part 1 Linguagem: Inglês

10.1016/s0022-5347(01)69118-3

1527-3792

Helga Habermann, Vera Ray, Walter Habermann, Gail S. Prins,

Mass Spectrometry Techniques and Applications

No AccessJournal of UrologyErratum1 Feb 2002ALTERATIONS IN GAP JUNCTION PROTEIN EXPRESSION IN HUMAN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCERis correction ofALTERATIONS IN GAP JUNCTION PROTEIN EXPRESSION IN HUMAN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCER HELGA HABERMANN, VERA RAY, WALTER HABERMANN, and GAIL S. PRINS HELGA HABERMANNHELGA HABERMANN , VERA RAYVERA RAY , WALTER HABERMANNWALTER HABERMANN , and GAIL S. PRINSGAIL S. PRINS View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)69118-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Gap junctions composed of connexin proteins have an essential role in intercellular communication and differentiation. Dysregulation of connexin expression is believed to have a role in carcinogenesis. The human prostate has been reported to express connexin 32 and 43. However, the expression pattern in prostate cancer is controversial, while to our knowledge connexin expression has not been reported in benign prostatic hyperplasia (BPH). To understand the potential involvement in prostate disease connexin 32 and 43 expression was evaluated in a series of normal prostate, BPH and prostate cancer specimens that were surgically removed due to bladder outlet obstruction. Materials and Methods: Frozen sections of 23 normal, 43 BPH and 40 cancer involved prostates were evaluated for the presence, staining intensity and pattern of connexin 32 and 43 by immunocytochemical testing. Results: In all specimens examined connexin 43 stain was punctate along the borders of the basal epithelial cells, whereas connexin 32 immunolocalized to luminal epithelial cells. In normal prostate connexin 43 and 32 were present in 87% and 65% of specimens, respectively, at low to moderate stain intensity. Importantly none of the normal samples were negative foreach connexin. In BPH specimens there was a marked increase in the incidence and intensity of connexin 43 and 32 immunostaining within epithelial cells. In addition, 23% of BPH samples showed strong connexin 43 expression in stromal cells. In contrast, connexin was decreased in prostate cancer specimens, of which 65% and 38% were negative for connexin 43 and 32, respectively, and 28% were negative for each type. In poorly differentiated tumors connexin 43 and 32 were present in only 10% and 40% of tumors, respectively, at low immunostaining intensity. Conclusions: In normal human prostate basal cells communicate via connexin 43 gap junctions, whereas luminal cells communicate via connexin 32 gap junctions. In BPH gap junctional intercellular communication is increased in epithelial and stromal cells, which may have a role in BPH pathogenesis. 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Related articlesJournal of Urology9 Nov 2018ALTERATIONS IN GAP JUNCTION PROTEIN EXPRESSION IN HUMAN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCER Volume 167Issue 2 Part 1February 2002Page: 655-660 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsprostateprostatic neoplasmsprostatic hyperplasiaconnexinsMetrics Author Information HELGA HABERMANN More articles by this author VERA RAY More articles by this author WALTER HABERMANN More articles by this author GAIL S. PRINS Requests for reprints: Department of Urology, M/C 955, University of Illinois, Chicago, Illinois 60612. More articles by this author Expand All Advertisement PDF downloadLoading ...