Agrobacterium tumefaciens -mediated transformation of Paracoccidioides brasiliensis
2004; Oxford University Press; Volume: 42; Issue: 4 Linguagem: Inglês
10.1080/13693780410001712007
ISSN1460-2709
AutoresClaudia Leal, Beatriz Montes, Ana Cecilia Mesa, Álvaro León Rua, Mauricio Corredor, Ángela Restrepo, Juan G. McEwen,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoParacoccidioidesbrasiliensis is the causative agent of paracoccidioidomycosis, an important mycosis endemic to Latin America. As the tools to study gene function in P.brasiliensis are only in the early stage of development, there is presently no system that allows for both the delivery and integration of exogenous nucleic acids into its genome. We report in this paper the transformation of the yeast phase of P.brasiliensis (ATCC-60855) with Agrobacteriumtumefaciens (GV3101) carrying the vector pAD1625. The microorganisms were co-cultivated for 2 days and then incubated for 10 days at 35°C on selective media. PCR and dot-blot targeted at a fragment of 222 bp from the hph (hygromycin phosphotransferase) gene which confers Hygr confirmed the transformation of P.brasiliensis.
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