Production of monoclonal antibodies for the detection of potato virus Y
1986; Wiley; Volume: 109; Issue: 2 Linguagem: Inglês
10.1111/j.1744-7348.1986.tb05323.x
ISSN1744-7348
Autores Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoSUMMARY Monoclonal antibodies (McAb) were obtained to potato virus Y (PVY) after immunisation of BALB/c mice with purified PVY, tobacco necrotic strain (PVY n ). Spleen cells from a mouse showing a high serum titre were used for fusion with X63NS1 myeloma cells. Hybridomas were selected in medium containing HAT. Culture supernatants were screened for antibody production against PVY, ordinary strain (PVY 0 ) and PVY n using indirect ELISA. Clones of interest were further cross‐reacted with 12 isolates each of PVY 0 and PVY n and two isolates of potato virus A (PVA) and healthy sap. For further trials, two clones which reacted specifically with PVY n isolates and one which detected all PVY isolates except two of potato virus C (PVC) were selected.
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