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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

2010; Oxford University Press; Volume: 186; Issue: 2 Linguagem: Inglês

10.1534/genetics.110.120717

1943-2631

Michelle Christian, Tomáš Čermák, Erin Doyle, Clarice Schmidt, Feng Zhang, Aaron W. Hummel, Adam J. Bogdanove, Daniel F. Voytas,

Advanced biosensing and bioanalysis techniques

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.