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The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and of interleukin 2 responsiveness by concanavalin A are accessory cell dependent

1983; Wiley; Volume: 13; Issue: 1 Linguagem: Inglês

10.1002/eji.1830130103

1521-4141

Thomas Hünig, Michael Loos, Anneliese Schimpl,

Immunotherapy and Immune Responses

Abstract Recent studies from other laboratories have shown that concanavalin A (Con A) acts at two separate steps in polyclonal T cell activation: interleukin 2 (IL2) production, and induction of responsiveness to IL2. Using a combination of techniques for the depletion of accessory cells from lymph node T cells, we have investigated which of these steps, if not both, is responsible for the known requirement for accessory cells in the Con A response. It was found that with increasing T cell purification, first the ability is lost to produce sufficient levels of endogenous IL2, whereas induction of IL2 responsiveness can still take place. Further removal of accessory cells however yields a population of resting T cells that cannot be induced by Con A to become IL2‐reactive. It was concluded that both IL2 production and induction of reactivity to IL 2 are accessory cell‐dependent events.