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Dendrimer‐Functionalized Iron Oxide Nanoparticles for Specific Targeting and Imaging of Cancer Cells

2007; Wiley; Volume: 17; Issue: 16 Linguagem: Inglês

10.1002/adfm.200601139

1616-3028

S. H. Wang, Xiangyang Shi, Mary Van Antwerp, Zeyuan Cao, Scott D. Swanson, Xiangdong Bi, James R. Baker,

RNA Interference and Gene Delivery

Abstract We demonstrated a unique approach that combines a layer‐by‐layer (LbL) self‐assembly method with dendrimer chemistry to functionalize Fe 3 O 4 nanoparticles (NPs) for specific targeting and imaging of cancer cells. In this approach, positively charged Fe 3 O 4 NPs (8.4 nm in diameter) synthesized by controlled co‐precipitation of Fe II and Fe III ions were modified with a bilayer composed of polystyrene sulfonate sodium salt and folic acid (FA)‐ and fluorescein isothiocyanate (FI)‐functionalized poly(amidoamine) dendrimers of generation 5 (G5.NH 2 ‐FI‐FA) through electrostatic LbL assembly, followed by an acetylation reaction to neutralize the remaining surface amine groups of G5 dendrimers. Combined flow cytometry, confocal microscopy, transmission electron microscopy, and magnetic resonance imaging studies show that Fe 3 O 4 /PSS/G5.NHAc‐FI‐FA NPs can specifically target cancer cells overexpressing FA receptors. The present approach to functionalizing Fe 3 O 4 NPs opens a new avenue to fabricating various NPs for numerous biological sensing and therapeutic applications.