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DNA barcoding in medicinal plants Caprifoliaceae

2010; Chinese Pharmaceutical Association; Linguagem: Inglês

10.4268/cjcmm20101906

1001-5302

Zhen Liu,

Phytochemistry and Biological Activities

To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.