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RNA-sequencing from single nuclei

2013; National Academy of Sciences; Volume: 110; Issue: 49 Linguagem: Inglês

10.1073/pnas.1319700110

1091-6490

Rashel V. Grindberg, Joyclyn Yee-Greenbaum, Michael J. McConnell, Mark Novotny, A. O'Shaughnessy, Georgina M. Lambert, Marcos J. Araúzo‐Bravo, Jun Lee, Max Fishman, Gillian E. Robbins, Xiaoying Lin, Pratap Venepally, Jonathan H. Badger, David W. Galbraith, Fred H. Gage, Roger S. Lasken,

RNA modifications and cancer

Significance One of the central goals of developmental biology and medicine is to ascertain the relationships between the genotype and phenotype of cells. Single-cell transcriptome analysis represents a powerful strategy to reach this goal. We advance these strategies to single nuclei from neural progenitor cells and dentate gyrus tissue, from which it is very difficult to recover intact cells. This provides a unique means to carry out RNA sequencing from individual neurons that avoids requiring isolation of single-cell suspensions, eliminating potential changes in gene expression due to enzymatic-cell dissociation methods. This method will be useful for analysis of processes occurring in the nucleus and for gene-expression studies of highly interconnected cells such as neurons.