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Lactose hydrolysis by immobilized β‐galactosidase in capillary bed reactor

1989; Wiley; Volume: 34; Issue: 4 Linguagem: Inglês

10.1002/bit.260340404

1097-0290

Ronnie Peterson, C.G. Hill, Clyde H. Amundson,

Microbial Metabolic Engineering and Bioproduction

The hydrolysis of lactose by immobilized beta-galactosidase was studied in a continuous-flow capillary bed reactor operating at 30 degrees C. Solutions containing 50, 100, and 150 g lactose and 0.5 g sodium acetate/L were fed to the reactor. Lactose conversions ranging from 24% to greater than 99% were achieved at reactor space times ranging from 0.06 to 6.3 min. These conversion data were successfully modeled in terms of a plug flow reactor model and a form of Michaelis-Menten kinetics which included competitive inhibition by both the alpha and beta forms of galactose.