Molecularly Imprinted Electrochemical Luminescence Sensor Based on Enzymatic Amplification for Ultratrace Isoproturon Determination
2012; Wiley; Volume: 24; Issue: 7 Linguagem: Inglês
10.1002/elan.201200088
ISSN1521-4109
AutoresShuhuai Li, Huilin Tao, Jianping Li,
Tópico(s)Electrochemical Analysis and Applications
ResumoAbstract A new molecularly imprinted electrochemical luminescence sensor (MIP‐ECL sensor) was developed for isoproturon (IPU) determination based on the competition reaction between IPU and glucose oxidase labeled IPU (GOD‐IPU). After competition, hydrogen peroxide produced by residual GOD‐IPU on the MIP reacted with luminol to emit electrochemiluminescence (ECL) signal. The ECL intensity decreased when the GOD‐IPU molecules were replaced by IPU molecules in the samples. IPU could be determined in the concentration range from 9×10 −11 mol/L to 5.1×10 −9 mol/L with a detection limit of 3.78×10 −12 mol/L. Water samples were assayed and recoveries ranging from 98.5 % to 102.1 % were obtained.
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