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Pretreatment of indian cane molasses for increased production of citric acid

1984; Wiley; Volume: 26; Issue: 9 Linguagem: Inglês

10.1002/bit.260260915

1097-0290

Subir Kundu, T. Panda, S. K. Majumdar, B.K. Guha, K. K. Bandyopadhyay,

Enzyme Catalysis and Immobilization

Abstract Surface culture citric acid fermentation was carried out by Aspergillus niger T55, a strain isolated from its natural source, using cane molasses, either untreated or treated by various methods. Citric acid biosynthesis is seriously impaired by both organic and inorganic inhibitors. A combined treatment of molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation minimizes the level of inorganic and organic inhibitors in molasses and increases the production of citric acid (65% weight yield based on total reducing sugar). The optimum level of individual metal ions for citric acid production depends on the concentration of other metals in the medium.