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Artigo Acesso aberto Revisado por pares
BIBTEX RIS

Rapid Extraction of DNA From Escherichia coli and Cryptosporidium parvum for Use in PCR

2001; American Society for Microbiology; Volume: 67; Issue: 11 Linguagem: Inglês

10.1128/aem.67.11.5321-5324.2001

ISSN

1098-5336

Autores

James Higgins, Mark C. Jenkins, Daniel R. Shelton, R. Fayer, Jeffrey S. Karns,

Tópico(s)

Enterobacteriaceae and Cronobacter Research

Resumo

ABSTRACT The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum . All methods provided satisfactory DNA from E. coli , and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum .

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