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Correlation of HPLC and AOAC Methods to Assess the All-trans-Lutein Content in Marigold Flowers

1996; Wiley; Volume: 72; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-0010(199611)72

1097-0010

Francisco Delgado‐Vargas, Octavio Paredes‐López,

Phytochemicals and Antioxidant Activities

Journal of the Science of Food and AgricultureVolume 72, Issue 3 p. 283-290 Research Article Correlation of HPLC and AOAC Methods to Assess the All-trans-Lutein Content in Marigold Flowers Francisco Delgado-Vargas, Francisco Delgado-Vargas Depto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoSearch for more papers by this authorOctavio Paredes-López, Corresponding Author Octavio Paredes-López Depto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoDepto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoSearch for more papers by this author Francisco Delgado-Vargas, Francisco Delgado-Vargas Depto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoSearch for more papers by this authorOctavio Paredes-López, Corresponding Author Octavio Paredes-López Depto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoDepto de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apdo Postal 629, 36500 Irapuato, Gto, MexicoSearch for more papers by this author First published: November 1996 https://doi.org/10.1002/(SICI)1097-0010(199611)72:3 3.0.CO;2-VCitations: 32AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract A reversed-phase C30 high-performance liquid chromatography (HPLC) method was used to separate the lutein isomers of marigold flowers. The pigments were extracted from marigold meal by an Association of Official Analytical Chemists (AOAC) modified method. The unsaponified extract showed the presence of several lutein ester isomers and after saponification four major lutein isomers were detected. The relationship between all-trans-lutein, assessed by HPLC, and total xanthophylls, assessed by AOAC, was determined. A regression equation, with a high correlation coefficient (r2=0·972), which allows for the calculation of all-trans-lutein from total xanthophylls content is proposed. Citing Literature Volume72, Issue3November 1996Pages 283-290 RelatedInformation