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The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, d- and l-methionine

2003; Elsevier BV; Volume: 155; Issue: 2 Linguagem: Inglês

10.1016/j.resmic.2003.11.008

1769-7123

Marie-Françoise Hullo, Sandrine Auger, Elie Dassa, Antoine Danchin, Isabelle Martin‐Verstraete,

RNA and protein synthesis mechanisms

The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 microM l-methionine or 100 microM methionine sulfoxide, while it grows similarly to the wild type with 100 microM l-methionine and 1 mM methionine sulfoxide. Other uptake systems are therefore present for these two compounds. In contrast, the Yus ABC transporter corresponds to the sole d-methionine uptake system. We propose to rename yusC, yusB and yusA as metN, metP and metQ, respectively.