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Primary structure of the oligosaccharide determinant of blood group Cad specificity.

1983; Elsevier BV; Volume: 258; Issue: 12 Linguagem: Inglês

10.1016/s0021-9258(18)32234-8

1083-351X

Dominique Blanchard, Jean‐Pierre Cartron, Bernard Fournet, Jean Montreuil, H. van Halbeek, J.F.G. Vliegenthart,

Blood groups and transfusion

Glycophorin A and B from Cad erythrocyte membranes are the carriers of the blood group Cad determinants.They are characterized by a significant increase in molecular mass, as compared to the corresponding glycophorins from control erythrocytes (Cartron, J.-p., and Blanchard, D. (1982) Biochem.J. 207, 497-504).Lipid-free glycophorin A, purified from Cad red cells, showed an increased GalNAc content in comparison to blood group B, Cad-negative, control cells.Alkaline-borohydride treatment of this Cad glycophorin A released as a predominant species a pentasaccharide; its structure was determined, by methylation analysis and by 500-MHz 'H-NMR studies, to be: GalNAcS(1+4) \ / \ / Gal@( 143) NeuAca(243) GalNAc-ol NeuAca(246) This novel oligosaccharide inhibited strongly the hemagglutination of Cad erythrocytes by the Dolichos biflorus lectin.It shares with the blood group Sd" determinant a terminal GalNAcB( 1+4)Ga46( 1+. ) sequence.~ ~ ~Cad is a rare human red cell antigen inherited as an autosomal dominant character (1).Cells with such an antigenic activity were first recognized as blood group 0 or B erythrocytes exhibiting an unexpectedly strong reactivity with the Dolichos biflorus lectin.Later, hemagglutination-inhibition of Cad red cells by D. bifbrus lectin has shown that Cad specificity requires the presence of GalNAc' (2, 31, a sugar also involved in blood groups A, Tn, Sd", P, and Forssman speci-