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Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

2009; Elsevier BV; Volume: 78; Issue: 1 Linguagem: Inglês

10.1016/j.mimet.2009.04.014

1872-8359

Malena Sandberg, Denise Schellmann, Gerda Brunhofer, Thomas Erker, Igor Busygin, Reko Leino, Pia Vuorela, Adyary Fallarero,

Antimicrobial Peptides and Activities

Staining of Staphylococcus aureus biofilms with 20 µM resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated.