An end-point method for estimation of the total antioxidant activity in plant material
1998; Wiley; Volume: 9; Issue: 4 Linguagem: Inglês
10.1002/(sici)1099-1565(199807/08)9
ISSN1099-1565
AutoresAntonio Caño, Josefa Hernández‐Ruíz, Francisco Garcı́a-Cánovas, Manuel Acosta, Marino B. Arnao,
Tópico(s)Phytochemicals and Antioxidant Activities
ResumoPhytochemical AnalysisVolume 9, Issue 4 p. 196-202 Original Paper An end-point method for estimation of the total antioxidant activity in plant material Antonio Cano, Antonio Cano Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorJosefa Hernández-Ruíz, Josefa Hernández-Ruíz Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorFrancisco García-Cánovas, Francisco García-Cánovas Departamento de Bioquímica y Biología Molecular -A-, Universidad de Murcia. 30100-Murcia, SpainSearch for more papers by this authorManuel Acosta, Manuel Acosta Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorMarino B. Arnao, Corresponding Author Marino B. Arnao marino@gaia.fcu.um.es Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainDepartamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this author Antonio Cano, Antonio Cano Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorJosefa Hernández-Ruíz, Josefa Hernández-Ruíz Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorFrancisco García-Cánovas, Francisco García-Cánovas Departamento de Bioquímica y Biología Molecular -A-, Universidad de Murcia. 30100-Murcia, SpainSearch for more papers by this authorManuel Acosta, Manuel Acosta Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this authorMarino B. Arnao, Corresponding Author Marino B. Arnao marino@gaia.fcu.um.es Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainDepartamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, 30100-Murcia, SpainSearch for more papers by this author First published: 21 December 1998 https://doi.org/10.1002/(SICI)1099-1565(199807/08)9:4 3.0.CO;2-WCitations: 243AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical can be generated by the enzymatic system formed by hydrogen peroxide and horseradish peroxidase. This ABTS radical (ABTS·+), a chromogen, is stable at room temperature but is unstable above 35°C and/or at pH values of above 7.5. Nevertheless, the most important factor in its stability is the ABTS/ABTS·+ concentration ratio in the medium. The radical reacts with the antioxidant, L-ascorbic acid, with a high rate constant, the stoichiometry of the reaction being 1 mol of L-ascorbic acid per 2 mol of ABTS·+ reduced. Based on these considerations, a spectrophotometric end-point method has been developed to evaluate L-ascorbic acid in aqueous media, and this represents an improvement over the lag-method previously reported. Under optimal conditions of temperature, pH and reagent concentration, the end-point method was capable of determining L-ascorbic acid with a limit of quantification of 0.38 nmol. In the assay described here, this ability is used to evaluate the total antioxidant activity of commercial citrus juices, in which ascorbic acid is a principal component. In our opinion this procedure can quickly provide useful information on the antioxidant content of foods and plant extracts. © 1998 John Wiley & Sons, Ltd. Citing Literature Volume9, Issue4July/August 1998Pages 196-202 RelatedInformation
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