Portal de Periódicos da CAPES

Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli

1979; Nature Portfolio; Volume: 281; Issue: 5733 Linguagem: Inglês

10.1038/281646a0

1476-4687

Francis Galibert, Elisabeth Mandart, Françoise Fitoussi, Pierre Tiollais, Patrick Charnay,

RNA and protein synthesis mechanisms

The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This sequence is 3,182 nucleotides long. Location of the nonsense codons shows that the coding capacity of the L chain is larger than the coding capacity of the S chain. Eight open regions, able to code for polypeptide chains larger than 100 amino acids, have been located. Region 6, which is the largest, covers more than 80% of the genome. The gene S which codes for polypeptide I of the Hbs Ag and was previously located between coordinates 95.1 and 73.6 is contained in region 7.