Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy

2013; Elsevier BV; Volume: 17; Issue: 11 Linguagem: Inglês

10.1016/j.ijid.2013.05.011

1878-3511

Paula Brito e Cabral, José Evandro Cunha Júnior, Alexandre Casimiro de Macedo, Alexandre Rodrigues Alves, Thially Braga Gonçalves, Tereza Cristina Brito e Cabral, Ana Paula Soares Gondim, Maria Isabel Moraes Pinto, Karen Oseki, Lília Maria Carneiro Câmara, Sílvia Helena Barem Rabenhorst, Aparecida Tiemi Nagao‐Dias,

Infectious Diseases and Tuberculosis

ObjectivesLeprosy household contacts represent a group at high risk of developing the disease. The aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.MethodsSerum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n = 135), their index cases (n = 30), and in persons living in a low endemic city (n = 17).ResultsSalivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p < 0.01, p < 0.005, and p < 0.0001, respectively). This was not observed for serum anti-PGL1 IgM (p > 0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p < 0.0001). For IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6%) and in three out of the 50 PB household leprosy contacts (6.0%).ConclusionWe strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts.