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URETHRAL AFFERENT NERVE ACTIVITY AFFECTS THE MICTURITION REFLEX; IMPLICATION FOR THE RELATIONSHIP BETWEEN STRESS INCONTINENCE AND DETRUSOR INSTABILITY

1999; Lippincott Williams & Wilkins; Volume: 162; Issue: 1 Linguagem: Inglês

10.1097/00005392-199907000-00069

1527-3792

SUK YOUNG JUNG, Matthew O. Fraser, Hideo Ozawa, Osamu Yokoyama, Mitsuharu Yoshiyama, William C. de Groat, Michael B. Chancellor,

Thermoregulation and physiological responses

No AccessJournal of UrologyInvestigative Urology1 Jul 1999URETHRAL AFFERENT NERVE ACTIVITY AFFECTS THE MICTURITION REFLEX; IMPLICATION FOR THE RELATIONSHIP BETWEEN STRESS INCONTINENCE AND DETRUSOR INSTABILITY SUK YOUNG JUNG, MATTHEW O. FRASER, HIDEO OZAWA, OSAMU YOKOYAMA, MITSUHARU YOSHIYAMA, WILLIAM C. DE GROAT, and MICHAEL B. CHANCELLOR SUK YOUNG JUNGSUK YOUNG JUNG , MATTHEW O. FRASERMATTHEW O. FRASER , HIDEO OZAWAHIDEO OZAWA , OSAMU YOKOYAMAOSAMU YOKOYAMA , MITSUHARU YOSHIYAMAMITSUHARU YOSHIYAMA , WILLIAM C. DE GROATWILLIAM C. DE GROAT , and MICHAEL B. CHANCELLORMICHAEL B. CHANCELLOR View All Author Informationhttps://doi.org/10.1097/00005392-199907000-00069AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: A causative relationship between stress urinary incontinence (SUI) and detrusor instability has been suspected but never proven. Many women with mixed incontinence have resolution of detrusor instability after surgical correction of SUI. We sought experimental support that stimulation of urethral afferent nerves can induce or change reflex detrusor contractions. Materials and Methods: Urethral perfusion pressure and isovolumetric bladder pressure were measured with catheters inserted through the bladder dome in urethane anesthetized female S.D. rats (250 to 300 grams; n = 12). The catheter assembly was seated securely in the bladder neck to block passage of fluid between the bladder and urethra without affecting the nerve supply to the organs. The external urethra was not catheterized. Responses were examined in the control state at a urethral saline perfusion speed of 0.075 ml. per minute. Intraurethral drugs were administered following blockade of striated sphincter activity with intravenous alpha-bungarotoxin (0.1 mg./kg.). Results: Stopping the urethral saline infusion caused a significant decrease in micturition frequency in approximately 50% of the animals studied (n = 12). Intraurethral lidocaine (1%) infused at 0.075 ml. per minute caused a slight decrease in urethral perfusion pressure but no change in detrusor contraction amplitude. However, intraurethral lidocaine caused a significant (45%) decrease in the bladder contraction frequency (n = 5). The micturition frequency returned to baseline 30 minutes after stopping lidocaine infusion. Intraurethral infusion of nitric oxide (NO) donors (S-nitroso-N-acetylpenicillamine [SNAP] (2 mM) or nitroprusside (1 mM) immediately decreased urethral perfusion pressure by 30 to 37% (n = 5). A 45 to 75% decrease (n = 5) in bladder contraction frequency was also seen, which was similar to that observed following lidocaine. Neither NO donor changed the amplitude of bladder contractions. 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Google Scholar From the Division of Urologic Surgery and Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania(Chancellor) Requests for reprints: Urologic Surgery, Suite 700 Kaufmann Building, 3471 Fifth Avenue, Pittsburgh, PA 15213.Supported by NIH grant RO1 HD30522.© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited ByEggermont M, Wyndaele J, Gillespie J and De Wachter S (2018) Response Properties of Urethral Distension Evoked Unifiber Afferent Potentials in the Lower Urinary TractJournal of Urology, VOL. 194, NO. 5, (1473-1480), Online publication date: 1-Nov-2015.Serati M, Braga A, Sorice P, Siesto G, Salvatore S and Ghezzi F (2018) Solifenacin in Women with De Novo Overactive Bladder after Tension-Free Obturator Vaginal Tape—Is it Effective?Journal of Urology, VOL. 191, NO. 5, (1322-1326), Online publication date: 1-May-2014.Lee K, Sharma S, Tuttle J and Steers W (2018) Origin and Characterization of Retrograde Labeled Neurons Supplying the Rat Urethra Using Fiberoptic Confocal Fluorescent Microscopy In Vivo and ImmunohistochemistryJournal of Urology, VOL. 184, NO. 4, (1550-1554), Online publication date: 1-Oct-2010.Furuta A, Asano K, Egawa S, de Groat W, Chancellor M and Yoshimura N (2018) Role of α2-Adrenoceptors and Glutamate Mechanisms in the External Urethral Sphincter Continence Reflex in RatsJournal of Urology, VOL. 181, NO. 3, (1467-1473), Online publication date: 1-Mar-2009.Christ G, Bushman W and Fraser M (2009) Impact of Diabetes and Obesity on the Prostate and Urethra: Implications to Improved Bladder Dysfunction Understanding and TreatmentJournal of Urology, VOL. 182, NO. 6S, (S38-S44), Online publication date: 1-Dec-2009.Yokoyama O, Yusup A, Oyama N, Aoki Y, Miwa Y and Akino H (2018) Improvement in Bladder Storage Function by Tamsulosin Depends on Suppression of C-Fiber Urethral Afferent Activity in RatsJournal of Urology, VOL. 177, NO. 2, (771-775), Online publication date: 1-Feb-2007.Kenton K, Simmons J, FitzGerald M, Lowenstein L and Brubaker L (2018) Urethral and Bladder Current Perception Thresholds: Normative Data in WomenJournal of Urology, VOL. 178, NO. 1, (189-192), Online publication date: 1-Jul-2007.Yokoyama O, Miwa Y, Oyama N, Aoki Y, Ito H and Akino H (2018) Antimuscarinic Drug Inhibits Detrusor Overactivity Induced by Topical Application of Prostaglandin E2 to the Urethra With a Decrease in Urethral PressureJournal of Urology, VOL. 178, NO. 5, (2208-2212), Online publication date: 1-Nov-2007.TORIMOTO K, HIRAO Y, MATSUYOSHI H, de GROAT W, CHANCELLOR M and YOSHIMURA N (2018) α1-ADRENERGIC MECHANISM IN DIABETIC URETHRAL DYSFUNCTION IN RATSJournal of Urology, VOL. 173, NO. 3, (1027-1032), Online publication date: 1-Mar-2005.SEKI S, SASAKI K, IGAWA Y, NISHIZAWA O, CHANCELLOR M, de GROAT W and YOSHIMURA N (2018) Suppression of Detrusor-Sphincter Dyssynergia by Immunoneutralization of Nerve Growth Factor in Lumbosacral Spinal Cord in Spinal Cord Injured RatsJournal of Urology, VOL. 171, NO. 1, (478-482), Online publication date: 1-Jan-2004.GIANNANTONI A, MEARINI E, Di STASI S, MEARINI L, BINI V, PIZZIRUSSO G and PORENA M (2018) ASSESSMENT OF BLADDER AND URETHRAL SPHINCTER FUNCTION BEFORE AND AFTER RADICAL RETROPUBIC PROSTATECTOMYJournal of Urology, VOL. 171, NO. 4, (1563-1566), Online publication date: 1-Apr-2004.TORIMOTO K, FRASER M, HIRAO Y, DE GROAT W, CHANCELLOR M and YOSHIMURA N (2018) Urethral Dysfunction in Diabetic RatsJournal of Urology, VOL. 171, NO. 5, (1959-1964), Online publication date: 1-May-2004.GUSTAFSON K, CREASEY G and GRILL W (2018) A Catheter Based Method to Activate Urethral Sensory Nerve FibersJournal of Urology, VOL. 170, NO. 1, (126-129), Online publication date: 1-Jul-2003.YOSHIMURA N and CHANCELLOR M (2018) Current and Future Pharmacological Treatment for Overactive BladderJournal of Urology, VOL. 168, NO. 5, (1897-1913), Online publication date: 1-Nov-2002.DE WACHTER S and WYNDAELE J (2018) DOES BLADDER TONE INFLUENCE SENSATION OF FILLING AND ELECTRO-SENSATION IN THE BLADDER? A BLIND CONTROLLED STUDY IN YOUNG HEALTHY VOLUNTEERS USING BETHANECHOLJournal of Urology, VOL. 165, NO. 3, (802-804), Online publication date: 1-Mar-2001. Volume 162Issue 1July 1999Page: 204-212 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information SUK YOUNG JUNG More articles by this author MATTHEW O. FRASER More articles by this author HIDEO OZAWA More articles by this author OSAMU YOKOYAMA More articles by this author MITSUHARU YOSHIYAMA More articles by this author WILLIAM C. DE GROAT More articles by this author MICHAEL B. CHANCELLOR More articles by this author Expand All Advertisement PDF DownloadLoading ...