Produção Nacional
Revisado por pares
Average protein density is a molecular‐weight‐dependent function
2004; Wiley; Volume: 13; Issue: 10 Linguagem: Inglês
10.1110/ps.04688204
ISSN1469-896X
AutoresHannes Fischer, Igor Polikarpov, A. F. Craievich,
Tópico(s)Protein purification and stability
ResumoAbstract The mass density of proteins is a relevant basic biophysical quantity. It is also a useful input parameter, for example, for three‐dimensional structure determination by protein crystallography and studies of protein oligomers in solution by analytic ultracentrifugation. We have performed a critical analysis of published, theoretical, and experimental investigations about this issue and concluded that the average density of proteins is not a constant as often assumed. For proteins with a molecular weight below 20 kDa, the average density exhibits a positive deviation that increases for decreasing molecular weight. A simple molecular‐weight‐depending function is proposed that provides a more accurate estimate of the average protein density.
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