Rab11-FIP3 and FIP4 interact with Arf6 and the Exocyst to control membrane traffic in cytokinesis
2005; Springer Nature; Volume: 24; Issue: 19 Linguagem: Inglês
10.1038/sj.emboj.7600803
ISSN1460-2075
AutoresAndrew B. Fielding, Eric Schonteich, Johanne Matheson, Gayle M. Wilson, Xinzi Yu, Gilles R.X. Hickson, Sweta Srivastava, Stephen A. Baldwin, Rytis Prekeris, Gwyn W. Gould,
Tópico(s)Pancreatic function and diabetes
ResumoArtilce8 September 2005free access Rab11-FIP3 and FIP4 interact with Arf6 and the Exocyst to control membrane traffic in cytokinesis Andrew B Fielding Andrew B Fielding Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Eric Schonteich Eric Schonteich Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Johanne Matheson Johanne Matheson Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Gayle Wilson Gayle Wilson Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Xinzi Yu Xinzi Yu Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Gilles RX Hickson Gilles RX Hickson Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UKPresent address: Department of Biochemistry and Biophysics, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA Search for more papers by this author Sweta Srivastava Sweta Srivastava School of Biochemistry and Microbiology, University of Leeds, Leeds, UK Search for more papers by this author Stephen A Baldwin Stephen A Baldwin School of Biochemistry and Microbiology, University of Leeds, Leeds, UK Search for more papers by this author Rytis Prekeris Corresponding Author Rytis Prekeris Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Gwyn W Gould Corresponding Author Gwyn W Gould Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Andrew B Fielding Andrew B Fielding Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Eric Schonteich Eric Schonteich Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Johanne Matheson Johanne Matheson Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Gayle Wilson Gayle Wilson Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Xinzi Yu Xinzi Yu Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Gilles RX Hickson Gilles RX Hickson Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UKPresent address: Department of Biochemistry and Biophysics, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA Search for more papers by this author Sweta Srivastava Sweta Srivastava School of Biochemistry and Microbiology, University of Leeds, Leeds, UK Search for more papers by this author Stephen A Baldwin Stephen A Baldwin School of Biochemistry and Microbiology, University of Leeds, Leeds, UK Search for more papers by this author Rytis Prekeris Corresponding Author Rytis Prekeris Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA Search for more papers by this author Gwyn W Gould Corresponding Author Gwyn W Gould Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK Search for more papers by this author Author Information Andrew B Fielding1,‡, Eric Schonteich2,‡, Johanne Matheson1, Gayle Wilson2, Xinzi Yu1, Gilles RX Hickson1, Sweta Srivastava3, Stephen A Baldwin3, Rytis Prekeris 2 and Gwyn W Gould 1 1Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK 2Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, Aurora, CO, USA 3School of Biochemistry and Microbiology, University of Leeds, Leeds, UK ‡These authors contributed equally to this work *Corresponding authors: Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Centre, 12801 E. 17th Avenue, Aurora, CO 80045, USA. Tel.: +1 303 724 3411; Fax: +1 303 724 3420; E-mail: [email protected] Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK. Tel.: +44 141 330 5263; Fax: 44 141 330 4620; E-mail: [email protected] The EMBO Journal (2005)24:3389-3399https://doi.org/10.1038/sj.emboj.7600803 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The dual Rab11/Arf binding proteins, family of Rab11-interacting proteins FIP3 and FIP4 function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis. Here, we report that both FIP3 and FIP4 bind Arf6 in a nucleotide-dependent manner but exhibit differential affinities for Rab11 and Arf6. Both FIP3 and FIP4 can form ternary complexes with Rab11 and Arf6. Arf6 is localised to the furrow and midbody and we show that Arf6-GTP functions to localise FIP3 and FIP4 to midbodies during cytokinesis. Exo70p, a component of the Exocyst complex, also localises to the furrow of dividing cells and interacts with Arf6. We show that depletion of Exo70p leads to cytokinesis failure and an impairment of FIP3 and Rab11 localisation to the furrow and midbody. Moreover, Exo70p co-immunoprecipitates FIP3 and FIP4. Hence, we propose that FIP3 and FIP4 serve to couple Rab11-positive vesicle traffic from recycling endosomes to the cleavage furrow/midbody where they are tethered prior to fusion events via interactions with Arf6 and the Exocyst. Introduction A crucial facet of mammalian cell division is the separation of two daughter cells by a process known as cytokinesis. An early event in cytokinesis is the formation of an acto-myosin contractile ring, which functions like a purse string in the constriction of the forming furrow between the two cells (Glotzer, 2001). Far less well characterised are the membrane trafficking steps, which deliver new membrane to the cell surface during the plasma membrane expansion known to accompany furrow formation (O'Halloran, 2000). It is now clearly established that the plasma membrane at the cleavage furrow of mammalian cells has a distinct lipid and protein composition to the rest of the plasma membrane (Emoto et al, 1996; Emoto and Umeda, 2000; O'Halloran, 2000; Finger and White, 2002). This may reflect a requirement both for increased surface area during furrowing and for the coordinated delivery of intracellular signalling or membrane remodelling activities to the correct spatial coordinates during cleavage. Understanding the molecular basis and regulation of these trafficking events is crucial to a full understanding of cell division. We recently provided evidence showing that Rab11 is required for mammalian cell cytokinesis (Wilson et al, 2005), supporting and extending previous observations in both Drosophila melanogaster and Caenorhabditis elegans (Skop et al, 2001; Pelissier et al, 2003; Riggs et al, 2003). By cycling between GTP- and GDP-loaded conformations, Rab proteins function to recruit different effector proteins that regulate aspects of membrane function including trafficking and membrane fusion (Zerial and McBride, 2001). Our identification of a role for Rab11 in cytokinesis suggests that at least some of the new membrane incorporated into the plasma membrane during furrow expansion is derived from recycling endosomes. Recently, we and others have identified a novel family of Rab11-interacting proteins (FIPs) (Shin et al, 1999; Prekeris et al, 2000, 2001; Hales et al, 2001; Hickson et al, 2003), which all share a highly conserved, 20-amino-acid motif at the C-terminus of the protein, known as the Rab11-binding domain (RBD) (Prekeris et al, 2001; Meyers and Prekeris, 2002). Class II FIPs (Rab11-FIP3 and Rab11-FIP4) are characterised by the presence of EF hands and by a degree of homology with the Drosophila protein Nuclear Fallout, a protein required for cellularisation of Drosophila embryos (Rothwell et al, 1998; Riggs et al, 2003). Consistent with this, we observed that Rab11-FIP3/Arfophilin (hereafter FIP3) and Rab11-FIP4/Arfophilin-2 (hereafter FIP4) also play a role in cytokinesis (Wilson et al, 2005). Rab11 recruits FIP3 to endosomes that accumulate in the furrow region during telophase, and mutants of FIP3 that cannot bind Rab11 no longer associate with recycling endosomes. Such mutants, and also knock down of FIP3 expression with RNAi, result in defective cytokinesis (Wilson et al, 2005). We have shown that Rab11-/FIP3-positive vesicles derived from recycling endosomes traffic to the furrow via centrosome-anchored microtubules where they function to deliver membrane during furrow ingression and abscission as well as possibly to regulate actin remodelling (Wilson et al, 2005). Consistent with this model, both FIP3 and FIP4 are reported to bind Arf proteins (Hickson et al, 2003), monomeric GTPases that also regulate actin dynamics, and both FIP3 and FIP4 exhibit dynamic association with centrosomes (Wilson et al, 2005). These data, although implicating FIP3 and FIP4 in cytokinesis, leave several questions unanswered. Firstly, why do the class II FIPs interact with different GTPases? Secondly, what are the relative affinities for binding of these GTPases? Thirdly, although Rab11 recruits FIP3 to endosomes, it is not involved in FIP3 and FIP4 recruitment to the midbody, so how do these proteins become localised in this region? Finally, how can class II FIPs be integrated into a model of membrane vesicle docking and fusion in the furrow and/or midbody? Here, we set out to answer these questions. We determined the relative affinity of FIP3 and FIP4 for Rab11, Arf5 and Arf6, and assayed their ability to form ternary complexes with Rab and Arf proteins using recombinant bacterially expressed proteins. We show that FIP3 exhibits preferential binding of Rab11>Arf6≫Arf5. By contrast, FIP4 binds Arf6≫Rab11≃Arf5. Interestingly, both FIP3 and FIP4 are capable of binding Rab11 and Arf6 simultaneously, suggesting that the binding sites for these small GTPases are distinct and nonoverlapping. Mutants of FIP3 and FIP4 that have lost the ability to bind Rab11 but are still capable of interaction with Arf6 support such an interpretation. Using fluorescence microscopy, we previously demonstrated FIP3 in vesicles around the cleavage furrow during late telophase/early cytokinesis, and a recruitment of FIP3 to the midbody during cytokinesis (Wilson et al, 2005). Here, we show that FIP4 also exhibits strong staining in the midbody and on the spindle microtubules. We further show that both FIP3 and FIP4 are localised to midbodies by interaction with Arf6: a GTP-restricted mutant of Arf6 (Arf6-Q67L) recruits FIP3 to midbodies, and the normal localisation of endogenous FIP3 and FIP4 to the midbody of dividing cells is blocked by the GDP-restricted Arf6 mutant, Arf6-T27N. Previous work has shown that Arf6 interacts with the mammalian Exocyst complex (Prigent et al, 2003). Such data offer an intriguing model whereby Rab11-dependent recruitment of FIPs to recycling endosomes may endow these vesicles with the means to interact, via Arf6, with a tethering factor upon arrival at the furrow or midbody. As active Arf6 localises to the furrow and midbody (Schweitzer and D'Souza-Schorey, 2002), we hypothesised that the FIP–Arf6–Exocyst interaction may serve to tether recycling endosomes in the furrow prior to fusion. Consistent with this, we find that Exo70p, a component of the Exocyst complex, co-immunoprecipitates FIP3 and FIP4 and that depletion of Exo70p using small interfering RNA results in a profound cytokinesis defect. Hence, we propose that FIP3 and FIP4 serve to couple Rab11-positive vesicle traffic from recycling endosomes to the cleavage furrow/midbody where they are tethered prior to fusion events via interactions with Arf6 and the Exocyst. Results Biochemical characterisation of FIP3 and FIP4 interactions with GTPases We sought to determine the relative preference of Rab and ARF GTPases interactions with class II FIPs. To achieve this, we expressed the C-terminal domains of FIP3 (residues 300–759) and FIP4 (residues 227–557) as hexa–his fusion proteins in bacteria, and used these together with GST-Rab or GST-Arf proteins in a series of biochemical interaction studies (Figures 1 and 2). FIP3 was found to interact only with Rab11 (Figure 1A), exhibiting a roughly 100-fold greater affinity for Rab11 compared to the Rab family members tested (Rabs 3, 4, 5 (Figure 1A) 7, 9 and 22 (data not shown)). This is consistent with studies of other members of the FIP family of proteins (Junutula et al, 2004). We have previously shown that FIP3 can bind Arf6, and was originally identified in a yeast two-hybrid screen using Arf5-Q71L as bait (Hickson et al, 2003). Hence, we compared the ability of FIP3 to bind these different GTPases in vitro. Using a constant amount of GST-Arf or GST-Rab11, we performed a titration experiment to determine the relative affinities of FIP3 for these proteins. We found that FIP3 strongly interacted with Rab11, and also interacted with Arf6 (Figure 1B). The interaction of FIP3 with Arf5 was considerably less than that with Arf6. These data suggest a relative preference of interaction of FIP3 with Rab11>Arf6≫Arf5. Consistent with this, we found that FIP3 co-immunoprecipitated only Arf6 from transfected HeLa cells (Figure 1C). We then examined the ability of FIP3 to assemble ternary complexes with Rab11 and Arf6. As shown in Figure 1D and E, FIP3 forms a ternary complex with both these GTPases. Thus, Figure 1D shows that increasing concentrations of Rab11 are unable to displace GST-Arf6 from previously assembled FIP3 complexes, and Figure 1E shows that FIP3 in complex with Rab11 can associate with GST-Arf6. Collectively, these data argue that FIP3 can form a ternary complex with Arf6 and Rab11, at least in vitro. Figure 2 reveals a similar analysis for FIP4. This protein exhibited subtle differences in order of preference for the different GTPases, with Arf6 exhibiting the strongest binding, and Rab11 and Arf5 binding with similar affinities (Figure 2A). In data not shown here, we observed no interaction of FIP4 with Rab3, 4, 7, 9 or 22. FIP4 is also capable of forming a ternary complex with Rab11 and Arf6 in vitro (Figure 2B). Although we have previously shown a GTP-dependent interaction with Arf5 for both FIP3 and FIP4, the nucleotide dependence of Arf6 binding had not been addressed. To that end, Figure 2C shows that GTP-loaded wild-type Arf6-GST can pull down both FIP3 and FIP4, but that a dominant-negative mutant does not. As only Arf6 appears to interact with FIP3 and FIP4 in vivo, we have focused upon this interaction in the experiments below (see also Discussion). Figure 1.FIP3 binds Arf6 and Rab11. The binding characteristics of FIP3 were examined in a series of in vitro experiments. The C-terminus of FIP3 (residues 300–759) or Rip11 (residues 490–652) were expressed in bacteria as hexa–his fusions and incubated with 50 μl of beads loaded with 5 μg GST alone, or GST-Rabs/Arfs as indicated for 1 h. After washing, the beads were boiled in SDS–PAGE sample buffer and levels of associated FIP3 or Rip11 determined by immunoblotting. Data from a representative experiment is shown in (A). In the experiment shown in (B). increasing concentrations of FIP3-containing beads were incubated with 5 μg of Arf5, Arf6 or Rab11, and the binding determined by quantitative immunoblotting. The data shown is from three experiments of this type (mean±s.d.). (C) HeLa cells were transfected with HA-tagged Arf1, Arf5 or Arf6 as described. After 48 h, cells were lysed and an anti-HA immunoprecipitation performed. The immunoprecipitates were probed for the presence of endogenous FIP3. The result of a typical experiment is shown. In the experiment shown in (D), 50 μl of beads loaded with 5 μg of GST or GST-Arf6 were incubated with recombinant FIP3 in the presence of increasing amounts of Rab11. The ability of Rab11 to displace FIP3 from the Arf6-loaded beads was determined by immunoblotting. The experiment shown is typical of three experiments of this type. In order to determine whether FIP3 can form a ternary complex with Arf6 and Rab11, we performed the experiment shown in (E). GST-Arf6 was incubated with recombinant FIP3 (residues 300–759) or Rip11 (residues 490–652). The upper panel shows a Coomassie stain, revealing the interaction of FIP3 with Arf6, but not Rip11. These beads were incubated with recombinant Rab11, washed and then boiled in SDS–PAGE buffer and immunoblotted for Rab11 (middle panel) or Rip11 (lower panel). Download figure Download PowerPoint Figure 2.FIP4 has distinct GTPase preferences. Increasing concentrations of FIP4-containing beads (residues 227–557) were incubated with 5 μg of Arf5, Arf6 or Rab11, and the binding determined by quantitative immunoblotting. The data shown in (A) is from three experiments of this type, with the mean±s.d. shown. In order to determine whether FIP4 can form a ternary complex with Arf6 and Rab11, we performed the experiment shown in (B). GST-Arf6 was incubated with bacterially expressed FIP4 (residues 227–557) or Rip11 (residues 490–652). The upper panel shows a Coomassie stain demonstrating equivalent loads of Arf6 in each of the three conditions; the presence of FIP4 was demonstrated by immunoblotting (middle panel). These beads were incubated with bacterially expressed Rab11, washed and then boiled in SDS–PAGE buffer and immunoblotted for Rab 11 (lower panel). As shown, Rab11 was only recovered in the GST-Arf6 beads in the presence of FIP4. (C) GST, GST-Arf6 or GST-Arf6-T44N expressed in bacteria were loaded on glutathione–sepharose and incubated with either bacterially expressed FIP3 (upper panel) or FIP4 expressed in CHO cells (lower panel). The beads were washed and boiled in SDS–PAGE buffer and immunoblotted for FIP3 or FIP4 as indicated. Start refers to the starting material (100 ng of FIP3, or 50% of the start volume of cell lysate for FIP4). Data are representative of three experiments of this kind. (D) HeLa cells were transfected with HA-tagged Arf1, Arf5 or Arf6 as described. After 48 h, cells were lysed and an anti-HA immunoprecipitation performed. The immunoprecipitates were probed for the presence of endogenous FIP4. The result of a typical experiment is shown. Download figure Download PowerPoint The Arf6 and Rab11 binding sites are distinct We have recently identified and characterised a domain present in class I FIPs that mediate interaction with Rab11 (Meyers and Prekeris, 2002; Junutula et al, 2004). Based on this work, we created a mutation in FIP3 (I737E) that also displays an inability to bind Rab11 (Wilson et al, 2005). Here, we show that this mutant retains the ability to interact with Arf6, suggesting that the binding sites for these two small GTPases are distinct (Figure 3). Similarly, a mutation in the Rab11 binding site of FIP4 (D538A) abrogates Rab11 binding but not Arf6 binding (Figure 3). When expressed in interphase cells, a GFP fusion of this protein was unable to induce the collapse of recycling endosomes observed with wild-type FIP4, consistent with a loss of its ability to bind Rab11 (data not shown). These data, together with the experiments shown in Figures 1D, E and 2C, strongly suggest that the binding sites for Arf and Rab proteins on FIP3 and FIP4 are distinct. Figure 3.The Rab11 and Arf6 binding sites are spatially distinct. Beads loaded with GST, GST-FIP3 or GST-FIP3-I737E were incubated with either Rab11 or Arf6 as described. After incubation, the beads were washed and boiled in SDS–PAGE buffer and subjected to immunoblot analysis with anti-Arf6 (middle panel) or anti-Rab11 (lower panel). Similar data using GST-FIP4 constructs are shown in the adjacent panel. Download figure Download PowerPoint FIP3 and FIP4 localisation is controlled by GTPase interactions We next sought to determine whether these different GTPase interactions contribute to compartmentalisation of the proteins in dividing cells. First, we examined the localisation of endogenous Arf6 during mitosis (Figure 4). In agreement with previous studies (Schweitzer and D'Souza-Schorey, 2002), Arf6 was localised to the plasma membrane and intracellular vesicles in interphase cells (Figure 4). By late telophase/cytokinesis, strong Arf6 staining in the midbody was clearly evident. This pattern of Arf6 localisation is similar to that reported elsewhere (Schweitzer and D'Souza-Schorey, 2002). Figure 4.The distribution of Arf6 during the cell cycle. HeLa cells were plated onto glass coverslips and 12 h later fixed and stained with anti-Arf6 antibodies. Shown are representative images at different stages of the cell cycle. Arf6 staining is pseudo-coloured green, DNA is pseudo-coloured blue. Download figure Download PowerPoint We have previously shown that the accumulation of FIP3-positive vesicles in the furrow of dividing cells required Rab11 (Wilson et al, 2005). However, we also observed that a population of FIP3 localised to the midbody independently of Rab11 (Wilson et al, 2005). The strong localisation of Arf6 staining in the plasma membrane of the furrow and midbody, together with the ability of FIP3 and FIP4 to bind Arf6 revealed in Figures 1 and 2, suggested that Arf6 may be involved in the localisation of FIPs to the furrow or midbody. The data in Figure 5 support this hypothesis. Overexpression of the GTP restricted form of Arf6 (Arf6-Q67L) recruits FIP3 to the midbody (Figure 5A and B), and increased the amount of FIP4 present in this region (Figure 5D). Quantification of this data revealed that in cells expressing wild-type Arf6, FIP3 was present in the furrow of 92% (n=62) of cells; expression of Arf6-Q67L increased this to 96% (n=53). Similarly, FIP4 was present in the furrow or midbody of 91% (n=50) of cells expressing Arf6 wild type and 98.6% of cells expressing Arf6-Q67L (n=41). More strikingly however, Figure 5A shows that the association of FIP3 with the furrow or midbody is blocked by the expression of GDP-restricted Arf6-T27N mutant; similarly, localisation of FIP4 to the furrow/midbody was blocked by this mutant (Figure 5D). Analysis of three separate experiments of this type revealed no furrow or midbody FIP3 staining in 79% of cells expressing Arf6-T27N and no furrow or midbody FIP4 staining in 86% of such cells. We also examined the localisation of endogenous Rab11 in cells expressing these different Arf6 mutants (Figure 5D) as we have previously reported that Rab11-positive structures are localised within the furrow and midbodies of dividing cells (Figure 5C and Wilson et al, 2005). We consistently observed a reduction in the intensity of Rab11 staining in midbody region of cells overexpressing Arf6-T27N; however, overexpression of wild-type or constitutively active Arf6 had little effect. Finally, we used real-time image analysis to examine the trafficking of YFP-FIP3 in cells coexpressing either CFP-Rab11 or CFP-Arf6 (Figure 6; Supplementary movies 1 and 2). These tagged proteins have been shown by other studies to faithfully represent the distribution of the endogenous proteins (Aalto et al, 1993; Ashery et al, 1999; Aikawa and Martin, 2003). These data further support our hypothesis that Rab11 controls recruitment of FIP3 to endosomes, and further reveal that Arf6 appears at the midbody at a later stage of cytokinesis. Note that in the absence of overexpression of active Arf6, the fraction of total cellular FIP3 in the midbody is low, and cannot be clearly observed in the image series presented. However, the data in Figure 5A and B clearly reveal that FIP3 is present in the furrow and midbody, and is recruited to the midbody by active Arf6. Finally, we note that FIP4 exhibits similar spatial dynamics to FIP3, exhibiting clear recruitment to centrosomes prior to movement into the furrow and midbody (see Supplementary Figure S1). Figure 5.Arf6 controls midbody localisation of FIP3 and FIP4. (A) HeLa cell were cotransfected with GFP-FIP3 (green) and HA-Arf6, HA-Arf6-Q67L or HA-Arf6-T27N (red). Cells were fixed and imaged. Yellow represents the degree of the overlap. Note that expression of Arf6-Q67L results in a marked accumulation of FIP3 at the midbody, and that 79% of cells expressing Arf6-T27N exhibited no FIP3 in the midbody. (B) HeLa cells stably expressing GFP-FIP3 were transfected with either wild-type Arf6 or Arf6-Q67L. Shown are representative images of the FIP3 localisation in the furrow/midbody regions of such cells. Note that for clarity, the HA staining is not shown in panels B–D. (C) As panel A, except cells were stained for endogenous Rab11 distribution. (D) As panel A, except GFP-FIP4 was used. Note that FIP4 accumulation in the midbodies was abrogated by overexpression of Arf6-T27N. In all, >80% of cells expressing Arf6-T27N exhibited little or no FIP4 staining in the midbody. Download figure Download PowerPoint Figure 6.Real-time analysis of Rab11, Arf6 and FIP3 movement to the furrow and midbody. HeLa cells stably expressing either FIP3-YFP/CFP-Rab11 (A) or FIP3-YFP/Arf6-CFP (B) were plated on collagen-coated coverslips and imaged using time-lapse microscopy (see Supplementary movies 1 and 2). Images were collected every 1.4 min for 2.5 h. Note that Rab11 always colocalizes with FIP3, while Arf6 appears to be recruited directly to the midbody (asterisk). FIP3 at the midbody is a small fraction of the total cellular FIP3, and is not clearly evident in these nonconfocal images. Download figure Download PowerPoint FIP4 and Arf6 exhibit similar patterns of immunofluorescence staining at all stages of the cell cycle (Figure 7). This suggests that the ability of both FIP3 and FIP4 to bind Arf6 is mirrored by their localisation to midbodies. Such data suggest the hypothesis that Arf6 is involved in a late stage of vesicle docking at the midbody. Consistent with this interpretation, we and others have found a cytokinesis defect in cells expressing Arf6 mutants (data not shown; Schweitzer and D'Souza-Schorey, 2002). Figure 7.FIP4 exhibits a similar distribution to Arf6 during the cell cycle. Cells plated as outlined in Figure 5 were stained for FIP4 (red) or DNA (blue). The images shown are DeltaVision reconstructions and are typical of many images of this type. Scale bar 5 μm. Download figure Download PowerPoint FIP3 and FIP4 interact with the Exocyst Previous work has shown that Arf6 interacts with the mammalian Exocyst complex (Prigent et al, 2003). Such data offer an intriguing model whereby Rab11-dependent recruitment of FIPs to recycling endosomes may endow these vesicles with the means to interact, via Arf6, with a tethering factor upon arrival at the furrow or midbody. Consistent with an important role of the Exocyst complex in cytokinesis, immunostaining of dividing cells revealed localisation of Exo70p, a component of the Exocyst, to the cleavage furrow (Figure 8A). In order to ascertain whether Exo70p was important for cytokinesis, we utilised siRNA to knock down levels of this protein. Figure 8B shows that siRNA targeted to Exo70p effectively knocked down expression levels of this protein, but were without effect on levels of either Rab11 or FIP3. Knockdown of Exo70p resulted in a significant fraction of cells exhibiting a binuclear phenotype compared to controls (39.4±4.2 versus 8.1±2.9%, respectively; Figure 8C and D), consistent with an important role for Exo70p in cytokinesis. To test directly for an interaction of FIP3 or FIP4 with this protein, we performed co-immunoprecipitation experiments. GFP-tagged FIP3 or FIP4 were overexpressed in CHO cells, and antibodies against Exo70p were used to immunoprecipitate any potential complexes of these proteins. As shown in Figure 8E, Exo70p consistently pulled down GFP-FIP3 from cell lysates; Rab11 was also present in the pull-downs. Similar data were also observed using GFP-FIP4 lysate. Intere
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