MUTATIONS INDUCED IN ARABIDOPSIS BY DNA NUCLEOSIDE ANALOGS
1969; Volume: 61; Issue: 1 Linguagem: Inglês
10.1093/genetics/61.1.191
ISSN3049-7094
Autores Tópico(s)Plant tissue culture and regeneration
ResumoUBSTITUTION of purine and pyrimidine analogs for the normal bases of DNA has been used effectively for some time to induce mutations in bacteria and phages (FREESE 1963).In Neurospora 2-aminopurine increased mutation rate (BROCKMAN and DESERRES 1963).Among lower plants there is indirect evidence that 5-bromouracil caused the induction of plastid mutations in Euglena (SCHER and COLLINGE 1965).A single type of chloroplast mutant was found in progenies of two plants out of four that survived from 25 seeds of a commercial stock of Ageratum treated with 5-bromodeoxyuridine (ZAMECNIK and ZAMECNIK 1966).In higher plants various cytogenetic effects of DNA base analogs have been studied.Certain pyrimidine (SMITH and KUGELMAN 1961 ; SMITH, FUSSELL and KUGELMAN 1963; SMITH, KUGELMAN, COMMERFORD and SZYBALSKI 1963) and purine (SMITH 1961; SMITH and KUGELMAN 1964) analogs have been shown to be incorporated in the chromosomes of Vicia faba root tip cells at levels that did not cause aberrations.The halogen substituted analog 5-fluorodeoxyuridine induced chromosome gaps and fragments in cells of this plant ( TAYLOR, HAUT and TUNG 1962) although the breakage was independent of DNA synthesis (BELL and WOLFF 1964).The rather small scale experiments in Arabidopsis with bromouracil (ROB-BELEN 1964) and bromodeoxyuridine (MULLER 1965;JACOBS 1967) were considered negative by the authors though actually a few mutants were observed by MULLER.Striking differences in growth were induced by raising Arabidopsis plants on culture media containing up to 8 x 1 0-4 M 2-aminopurine or 5 x M 5-iododeoxyuridine (SMITH 1961 ) .The latter analog, when applied to premeiotic buds, was incorporated selectively into nuclei of gametophytic cells (BROWN and SMITH 1964), but distinct mutations affecting plant morphology were not recovered (BROWN, BHATIA and SMITH 1965).Though two independent thiaminerequiring mutants were found in 5-bromouracil-treated progenies of Arabidopsis, their frequency of occurrrence was as low as the expected spontaneous rate ( R ~D E I 1965).It has been demonstrated that 6-azapyrimidines ( R ~D E I 1965, 1967), 8- azaadenine ( HIRONO and RBDEI 1966a) and 5-halogen-substituted deoxycytidine and uridine (HIRONO and R ~D E I 1966b) modified the expression of mutant phenotypes of Arabidopsis when the plants were raised on analog-containing
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