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Enzymatic degradation of nitriles by a Candida guilliermondii UFMG-Y65

2000; Canadian Science Publishing; Volume: 46; Issue: 6 Linguagem: Inglês

10.1139/w00-027

1480-3275

João Carlos Teixeira Dias, Rachel Passos Rezende, Carlos A. Rosa, Marc‐André Lachance, Valter R. Linardi,

Microbial bioremediation and biosurfactants

Candida guilliermondii UFMG-Y65, isolated from a gold mine, was able to utilize different nitriles and the corresponding amides as sole source of nitrogen, at concentrations up to 2 M. Resting cells cultivated on YCB-acetonitrile medium showed nitrile hydrolyzing enzyme activities against acrylonitrile and benzonitrile. These enzymes were inducible and intracellular; the optimum pH was 7.0-8.0, and the optimum temperature 25°C-30°C. Liquid chromatographic analysis indicated that C. guilliermondii UFMG-Y65 metabolized 12 mM benzonitrile to 11 mM benzoic acid and 10 mM acrylonitrile to 7.9 mM acrylic acid. The results suggest that C. guilliermondii UFMG-Y65 may be useful for the bioproduction of amides and acids, and for the bioremediation of environments contaminated with nitriles.Key words: Candida guilliermondii, nitrile hydrolyzing enzyme, amidase, nitriles, amides.