Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
2010; Elsevier BV; Volume: 13; Issue: 4 Linguagem: Inglês
10.2225/vol13-issue4-fulltext-7
ISSN0717-3458
AutoresJosé Luis Piña-Escutia, Luis Miguel Vázquez-García, Amaury M. Arzate-Fernández,
Tópico(s)Advances in Cucurbitaceae Research
ResumoUn-weighted Pair Group Method Arithmetic Average Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México.This species is used as ornamental, food and medicinal purposes.Despite its ornamental and economic potential, there is little information about the genetic variability.In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavonia collected in three localities within State of México.The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Results showed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers.The
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