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MgATP Binding and Hydrolysis Determinants of NtrC, a Bacterial Enhancer-Binding Protein

1999; American Society for Microbiology; Volume: 181; Issue: 15 Linguagem: Inglês

10.1128/jb.181.15.4628-4638.1999

1098-5530

Irene Rombel, Petra Peters‐Wendisch, Andrew D. Mesecar, Thorgeir E. Thorgeirsson, Yeon‐Kyun Shin, Sydney Kustu,

DNA Repair Mechanisms

ABSTRACT When phosphorylated, the dimeric form of nitrogen regulatory protein C (NtrC) of Salmonella typhimurium forms a larger oligomer(s) that can hydrolyze ATP and hence activate transcription by the ς 54 -holoenzyme form of RNA polymerase. Studies of Mg-nucleoside triphosphate binding using a filter-binding assay indicated that phosphorylation is not required for nucleotide binding but probably controls nucleotide hydrolysis per se. Studies of binding by isothermal titration calorimetry indicated that the apparent K d of unphosphorylated NtrC for MgATPγS is 100 μM at 25°C, and studies by filter binding indicated that the concentration of MgATP required for half-maximal binding is 130 μM at 37°C. Filter-binding studies with mutant forms of NtrC defective in ATP hydrolysis implicated two regions of its central domain directly in nucleotide binding and three additional regions in hydrolysis. All five are highly conserved among activators of ς 54 -holoenzyme. Regions implicated in binding are the Walker A motif and the region around residues G355 to R358, which may interact with the nucleotide base. Regions implicated in nucleotide hydrolysis are residues S207 and E208, which have been proposed to lie in a region analogous to the switch I effector region of p21 ras and other purine nucleotide-binding proteins; residue R294, which may be a catalytic residue; and residue D239, which is the conserved aspartate in the putative Walker B motif. D239 appears to play a role in binding the divalent cation essential for nucleotide hydrolysis. Electron paramagnetic resonance analysis of Mn 2+ binding indicated that the central domain of NtrC does not bind divalent cation strongly in the absence of nucleotide.