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A NMR reverse diffusion filter for the simplification of spectra of complex mixtures and the study of drug receptor interactions

2011; Wiley; Volume: 49; Issue: 8 Linguagem: Inglês

10.1002/mrc.2786

1097-458X

Marino Vega-Vázquez, Juan Carlos Cobas, Francisco Fábio Oliveira de Sousa, Manuel Martín‐Pastor,

Advanced MRI Techniques and Applications

A reverse diffusion filter NMR experiment (Drev) is proposed for the study of small molecules in binding with macromolecules. The filtering efficiency of Drev to eliminate the signals of the macromolecule is shown to be superior to conventional transverse relaxation filters at least for macromolecules containing a significant fraction of flexible residues. The Drev filter was also a useful complement for ligand-based NMR screening in combination with saturation transfer difference experiments.