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A Molecular Dynamics Study on the Partitioning Mechanism in Hydrophilic Interaction Chromatography

2012; Wiley; Volume: 51; Issue: 25 Linguagem: Inglês

10.1002/anie.201201096

1521-3773

Sergey M. Melnikov, Alexandra Höltzel, Andreas Seidel‐Morgenstern, Ulrich Tallarek,

Protein purification and stability

Angewandte Chemie International EditionVolume 51, Issue 25 p. 6251-6254 Communication A Molecular Dynamics Study on the Partitioning Mechanism in Hydrophilic Interaction Chromatography Dr. Sergey M. Melnikov, Dr. Sergey M. Melnikov Max-Planck-Institut für Dynamik komplexer technischer Systeme, Sandtorstrasse 1, 39106 Magdeburg (Germany)Search for more papers by this authorDr. Alexandra Höltzel, Dr. Alexandra Höltzel Fachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekSearch for more papers by this authorProf. Dr. Andreas Seidel-Morgenstern, Prof. Dr. Andreas Seidel-Morgenstern Max-Planck-Institut für Dynamik komplexer technischer Systeme, Sandtorstrasse 1, 39106 Magdeburg (Germany)Search for more papers by this authorProf. Dr. Ulrich Tallarek, Corresponding Author Prof. Dr. Ulrich Tallarek tallarek@staff.uni-marburg.de Fachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekFachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekSearch for more papers by this author Dr. Sergey M. Melnikov, Dr. Sergey M. Melnikov Max-Planck-Institut für Dynamik komplexer technischer Systeme, Sandtorstrasse 1, 39106 Magdeburg (Germany)Search for more papers by this authorDr. Alexandra Höltzel, Dr. Alexandra Höltzel Fachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekSearch for more papers by this authorProf. Dr. Andreas Seidel-Morgenstern, Prof. Dr. Andreas Seidel-Morgenstern Max-Planck-Institut für Dynamik komplexer technischer Systeme, Sandtorstrasse 1, 39106 Magdeburg (Germany)Search for more papers by this authorProf. Dr. Ulrich Tallarek, Corresponding Author Prof. Dr. Ulrich Tallarek tallarek@staff.uni-marburg.de Fachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekFachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg (Germany) http://www.uni-marburg.de/fb15/ag-tallarekSearch for more papers by this author First published: 08 May 2012 https://doi.org/10.1002/anie.201201096Citations: 82Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The retention mechanism in hydrophilic interaction chromatography is supposed to involve partitioning of analytes between a mostly organic mobile phase and a water layer at the surface of the stationary phase. By molecular dynamics simulations, in which a 9 nm diameter bare silica pore is fed with H2O/CH3CN mixtures (see picture: 10:90 (v/v)), the water layer is elucidated and interpreted regarding the retention of hydrophilic analytes. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_201201096_sm_miscellaneous_information.pdf242 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume51, Issue25June 18, 2012Pages 6251-6254 RelatedInformation