Portal de Periódicos da CAPES

“Click-made” biaryl-linker improving efficiency in proteinlabelling for the membrane target protein of a bioactive compound

2010; Royal Society of Chemistry; Volume: 9; Issue: 1 Linguagem: Inglês

10.1039/c0ob00843e

1477-0539

Yoko Nakamura, Sho Inomata, Makoto Ebine, Yoshiyuki Manabe, Izumi Iwakura, Minoru Ueda,

Protein Degradation and Inhibitors

We report on the design, synthesis and assessment of a novel biaryl-linked (BArL) molecular probe for the exploration of low-abundant target proteins for bioactive compounds based on the activity based protein profiling (ABPP) approach. Surprisingly, the performance of the BArL probe was better than that of the stepwise tagging approach that is considered to be the most effective method used in ABPP study.