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<b>Development of a C-terminal-region-specific radioimunoassay of parathyroid hormone-related </b><b>protein </b>

1992; Allied Academies; Volume: 13; Issue: 2 Linguagem: Inglês

10.2220/biomedres.13.155

1880-313X

Hiroyuki Kasahara, M Tsuchiya, Ryoji Adachi, Shuji Horikawa, Shuichi Tanaka, Seiji Tachibana,

Parathyroid Disorders and Treatments

Few data are published regarding the molecular forms or concentrations of circulating and urinary parathyroid hormone-related protein (PTHrP) in normal subjects and patients with humoral hypercalcemia of malignancy (HHM).We have developed a C-terminal-regionspecific radioimmunoassay for human PTHrP] 09-141 (C-PTHrP radioimmunoassay) using a sheep antiserum immunized with a novel synthetic human PTHrP109-141 for immunogen and a novel synthetic [Tyr'08]PTHrP108-14] for radiolabel.The detection limit of this assay is 2 pM for human PTHrP109-141 and is sensitive enough to detect circulating and urinary C-PTHrP in normal subjects.The average immunoreactive concentration of serum (n=76) and urinary (11:12) C-PTHrP in normal subjects are 31.4iv 7.4 pM and 322.9 i 67.2 pM, respectively.Levels in patients with HHM are significantly elevated when compared to controls.This assay may be useful in the differential diagnosis of hypercalcemia.