In vivo activation of PPAR target genes by RXR homodimers
2004; Springer Nature; Volume: 23; Issue: 10 Linguagem: Inglês
10.1038/sj.emboj.7600209
ISSN1460-2075
AutoresAnnemieke IJpenberg, Nguan Soon Tan, Laurent Gelman, Sander Kersten, Josiane Seydoux, Jianming Xu, Daniel Metzger, Laurence Canaple, Pierre Chambon, Walter Wahli, Béatrice Desvergne,
Tópico(s)Nuclear Receptors and Signaling
ResumoArticle22 April 2004free access In vivo activation of PPAR target genes by RXR homodimers Annemieke IJpenberg Annemieke IJpenberg Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK Search for more papers by this author Nguan Soon Tan Nguan Soon Tan Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Laurent Gelman Laurent Gelman Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Sander Kersten Sander Kersten Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: Nutrition, Metabolism and Genomics Group, Wageningen University, Wageningen 6700 EV, the Netherlands Search for more papers by this author Josiane Seydoux Josiane Seydoux Centre Médical Universitaire, Département de Physiologie, Geneva, Switzerland Search for more papers by this author Jianming Xu Jianming Xu Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA Search for more papers by this author Daniel Metzger Daniel Metzger Institut de Génétique et de Biologie Moléculaire et Cellulaire/CNRS/INSERM/ULP/Collège de France, Illkirch, Strasbourg, France Search for more papers by this author Laurence Canaple Laurence Canaple Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: Laboratoire de Biologie Moléculaire et Cellulaire/UMR 5665, Ecole Normale Supérieure de Lyon, 46, Allée d'Italie, 69364 Lyon cedex 07, France Search for more papers by this author Pierre Chambon Pierre Chambon Institut de Génétique et de Biologie Moléculaire et Cellulaire/CNRS/INSERM/ULP/Collège de France, Illkirch, Strasbourg, France Search for more papers by this author Walter Wahli Walter Wahli Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Béatrice Desvergne Corresponding Author Béatrice Desvergne Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Annemieke IJpenberg Annemieke IJpenberg Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK Search for more papers by this author Nguan Soon Tan Nguan Soon Tan Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Laurent Gelman Laurent Gelman Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Sander Kersten Sander Kersten Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: Nutrition, Metabolism and Genomics Group, Wageningen University, Wageningen 6700 EV, the Netherlands Search for more papers by this author Josiane Seydoux Josiane Seydoux Centre Médical Universitaire, Département de Physiologie, Geneva, Switzerland Search for more papers by this author Jianming Xu Jianming Xu Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA Search for more papers by this author Daniel Metzger Daniel Metzger Institut de Génétique et de Biologie Moléculaire et Cellulaire/CNRS/INSERM/ULP/Collège de France, Illkirch, Strasbourg, France Search for more papers by this author Laurence Canaple Laurence Canaple Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, SwitzerlandPresent address: Laboratoire de Biologie Moléculaire et Cellulaire/UMR 5665, Ecole Normale Supérieure de Lyon, 46, Allée d'Italie, 69364 Lyon cedex 07, France Search for more papers by this author Pierre Chambon Pierre Chambon Institut de Génétique et de Biologie Moléculaire et Cellulaire/CNRS/INSERM/ULP/Collège de France, Illkirch, Strasbourg, France Search for more papers by this author Walter Wahli Walter Wahli Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Béatrice Desvergne Corresponding Author Béatrice Desvergne Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland Search for more papers by this author Author Information Annemieke IJpenberg1,‡, Nguan Soon Tan1,‡, Laurent Gelman1, Sander Kersten1, Josiane Seydoux2, Jianming Xu3, Daniel Metzger4, Laurence Canaple1, Pierre Chambon4, Walter Wahli1 and Béatrice Desvergne 1 1Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland 2Centre Médical Universitaire, Département de Physiologie, Geneva, Switzerland 3Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA 4Institut de Génétique et de Biologie Moléculaire et Cellulaire/CNRS/INSERM/ULP/Collège de France, Illkirch, Strasbourg, France ‡These authors contributed equally to this work *Corresponding author. Center for Integrative Genomics, NCCR Frontiers in Genetics, University of Lausanne, Biology Building, 1015 Lausanne, Switzerland. Tel.: +41 21 692 4140; Fax: +41 21 692 4115; E-mail: [email protected] The EMBO Journal (2004)23:2083-2091https://doi.org/10.1038/sj.emboj.7600209 Present address: MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. In contrast, the existence of an RXR/9-cis RA signalling pathway independent of PPAR or any other dimerization partner remains disputed. Using in vivo chromatin immunoprecipitation, we now show that RXR homodimers can selectively bind to functional PPREs and induce transactivation. At the molecular level, this pathway requires stabilization of the homodimer–DNA complexes through ligand-dependent interaction with the coactivator SRC1 or TIF2. This pathway operates both in the absence and in the presence of PPAR, as assessed in cells carrying inactivating mutations in PPAR genes and in wild-type cells. In addition, this signalling pathway via PPREs is fully functional and can rescue the severe hypothermia phenotype observed in fasted PPARα−/− mice. These observations have important pharmacological implications for the development of new rexinoid-based treatments. Introduction The family of nuclear hormone receptors plays a unique role in pathways involved in metabolic regulation by directly linking the presence or absence of signalling molecules to gene activation or repression. Within the nuclear receptor superfamily, a pivotal place is occupied by the retinoid X receptor (RXR). There are three RXR isotypes (RXRα, RXRβ and RXRγ), each encoded by a separate gene. All RXR isotypes bind with stereo-selectivity the vitamin A derivative 9-cis retinoic acid (RA). The physiological significance of the 9-cis RA signalling pathway is supported by the presence of 9-cis RA in the developing embryo and the identification of enzymes that contribute to its biosynthesis (Mertz et al, 1997; Romert et al, 1998). At the molecular level, several mechanisms are proposed for the biological action of 9-cis RA. First, 9-cis RA can bind to retinoic acid receptors (RARs) and activate the RAR:RXR heterodimer and the corresponding set of target genes. However, all-trans RA is a more abundant natural ligand for RAR. Second, in in vitro assays, 9-cis RA can induce the formation of RXR homodimers that bind to DR1 sequences (Zhang et al, 1992). It is so far unclear as to what extent this signalling mechanism is used in vivo, as few RXR-specific target genes have been identified. The third mechanism for 9-cis RA signalling is so far the best documented; it relies on the ability of RXR-containing heterodimers to mediate transcriptional activation in response to the RXR ligand (Mangelsdorf and Evans, 1995; Minucci and Ozato, 1996). Examples of such complexes include the heterodimers PPAR:RXR, LXR:RXR, NGF1B:RXR and also RAR:RXR. Among the heterodimers that can convey a 9-cis RA response, PPAR:RXR has been extensively studied and is considered an important mediator of both 9-cis RA action and of signalling by fatty acids and their derivatives (Desvergne and Wahli, 1999). Most of the ligand-dependent interactions of nuclear receptors and cofactors have been assessed for each receptor independently, and analysed in solution. In this context, the ligand-dependent interaction of PPAR on the one hand and of RXR on the other hand with the cofactor SRC1 is indeed well documented. Thus, the ability of PPAR:RXR to become transcriptionally active in the presence of either a PPAR or an RXR ligand has been interpreted as the ability of either receptor to recruit coactivator(s) (DiRenzo et al, 1997). It was recently shown that cofactors may interact differently with DNA-bound nuclear receptor heterodimers (Yang et al, 2000). To understand rexinoid action on genes controlled by a PPRE, we analysed coactivator recruitment by DNA-bound PPAR and RXR. Surprisingly, we found that DNA-bound PPAR:RXR does not interact with SRC1 in the presence of a PPAR ligand. In contrast, in the presence of an RXR agonist, SRC1 stabilizes the binding of a functional RXR homodimer to the PPRE. Using chromatin immunoprecipitation (ChIP) to assess complex formation both in primary keratinocyte cultures and in whole animal models, we show that RXR homodimers bind to PPREs via specific coactivator recruitment, irrespective of the presence of PPAR. Moreover, the resultant ternary complex is able to substitute functionally for PPAR:RXR and regulate complex metabolic pathways in vivo. Taken together, the data presented identify a new mechanism for RXR/9-cis RA signalling independent of a dimerization partner, in which the response element, through allosteric control of transcription factor/coactivator interactions, is able to discriminate functionally between competing signalling pathways. Results So far, the ligand-dependent interactions of PPAR, RXR or PPAR:RXR with coactivators have been assessed in solution and in the absence of DNA. As a first approach to determine how DNA-bound PPAR:RXR interacts with SRC1, we used electrophoretic mobility shift analysis (EMSA). As was shown previously (IJpenberg et al, 1997), recombinant PPARγ and RXR proteins form heterodimers that bind to the malic enzyme PPRE (MEp) (Figure 1A, lanes 2 and 3). Importantly, no recruitment of the SRC1 nuclear receptor interacting domain (SRC1(B); Jeannin et al, 1998) by DNA-bound PPAR:RXR is observed in the presence of the PPARγ ligand BRL49653 (Figure 1A, lanes 6 and 7). Similarly, no SRC1(B) recruitment was observed using PPARα and PPARβ with their appropriate ligands (data not shown). In contrast, in the presence of the RXR ligand 9-cis RA, a low-mobility complex binds to the PPRE, accompanied by a reduction in DNA-bound PPAR:RXR (Figure 1A, compare lanes 4, 6 and 8). In the presence of both PPAR and RXR ligands, a pattern similar to that of 9-cis RA alone is obtained (Figure 1A, compare lane 5 with 4, and lane 9 with 8). Remarkably, in the absence of PPAR (lanes 10–13), the same high-affinity low-mobility complex still binds to MEp in the presence of 9-cis RA. Furthermore, co-incubation with a polyclonal PPAR antibody (PPAR-Ab) that disrupts the PPAR:RXR interface reduces binding of PPAR:RXR but does not affect the complex formed in the presence of 9-cis RA and SRC1 (lanes 14–17). These results suggest that DNA-bound PPAR:RXR does not interact with SRC1 and that the observed ternary complex, comprising SRC1, may not contain PPAR. To assess directly the presence of SRC1 in the 9-cis RA-dependent ternary complex, DNA-dependent pull-down experiments were performed using either GST-SRC1(B) or full-length SRC1. Western blot analysis of the pulled-down protein complexes confirmed the tethering of SRC1 to MEp in the presence of RXR and its cognate ligand (Figure 1B, lanes 1–7, top two panels). As seen in the EMSA, the SRC1 pull-down was independent of the presence of PPAR (Figure 1B, lanes 8–13). Figure 1.Specific interaction of SRC1 with DNA-bound RXR:RXR complexes. (A) EMSA: Radiolabelled MEp was incubated with RXRα, PPARγ and SRC1(B), in the presence or absence of 1 μM 9-cis RA or 5 μM BRL49653, as indicated. The arrowheads indicate bound PPAR:RXR (grey), RXR:RXR (open) and ternary SRC1(B)-containing complex (black). Ab: polyclonal PPAR antibody; p.i.: preimmune serum. (B) Pull-down assays: Different combinations of RXRα, PPARγ, and either full-length SRC1 or the SRC1 nuclear receptor interacting domain (SRC1(B)) were incubated in 96-well plates coated with biotinylated DNA, either MEp-biotin or PDK1-biotin, in the presence of 40 μM 9-cis RA and/or 10 μM Rosiglitazone. After washing of the wells, retained proteins were analysed by Western blot with antibodies directed against GST (recognizing the GST-SRC1(B) or SRC1 (recognizing the full-length SRC1) as indicated. (C) EMSA: Analyses were performed as in (A), but in the absence of PPAR. DR1G: synthetic RXRE; MEp: functional PPRE from the malic enzyme promoter; AcoA: functional PPRE from the acyl-CoA oxidase promoter; HD: functional PPRE from the bifunctional enzyme promoter; MEd: nonfunctional DR1 element in the malic enzyme promoter. Download figure Download PowerPoint Although in vitro assays have shown that 9-cis RA can induce the binding of RXR homodimers to DR1-like elements (Nakshatri and Chambon, 1994), little if any binding has been seen with natural PPREs so far. In contrast, our results strongly suggest that SRC1 triggers the formation of a ternary complex composed of RXR homodimers and SRC1, which binds to MEp with a high affinity in the presence of 9-cis RA. As a positive control for RXR homodimer binding, we used the synthetic rexinoid response element DR1G (Nakshatri and Chambon, 1994). As expected, RXR homodimers efficiently bind to DR1G in the presence of 9-cis RA even in the absence of SRC1 (Figure 1C). Upon addition of SRC1(B), an RXR:RXR:SRC1(B) complex is formed, which migrates exactly like the ternary complex bound to MEp. We next assessed the specificity of RXR homodimer binding by either DNA-dependent pull-down analysis or EMSA, and found that the functional PPREs from the PDK1 promoter (Figure 1B, lower two panels) and from the acyl-CoA oxidase (AcoA) and the bifunctional enzyme (HD) promoters (Figure 1C, lanes 5–8) all tethered SRC1 in the presence of 9-cis RA and RXR. In contrast, RXR homodimers do not bind to the nonfunctional malic enzyme distal DR1-like element (MEd) (IJpenberg et al, 1997) under any of the conditions tested (Figure 1C, lanes 9 and 10). To further analyse RXR homodimer binding to the PPREs, EMSA was performed using the light-stable synthetic rexinoids LG100268 and LG100754. Whereas the first compound is a potent RXR pan-agonist, LG100754 has been characterized as an agonist of PPAR:RXR and RXR:VDR heterodimers, but an antagonist of RXR homodimers (Lala et al, 1996). Based on our results, we postulated that RXR:RXR would bind to PPREs only upon activation by LG100268, but not by LG100754. Indeed, the RXR:RXR/SRC1 ternary complex bound to the PPRE (MEp) as well as to the RXRE (DR1G) in the presence of either 9-cis RA or LG100268, but not in the presence of the RXR homodimer antagonist LG100754 (Figure 2A). These results were confirmed using full-length SRC1 in pull-down experiments (data not shown). Altogether, the results confirm that the ternary complexes binding to functional PPREs in response to RXR agonists are RXR homodimers stabilized by SRC1. As a first approach to assess the functionality of RXR homodimer binding to PPREs, we cotransfected NIH-3T3 cells with a reporter plasmid controlled by MEp (IJpenberg et al, 1997) and an RXR-encoding plasmid. As expected, reporter activity was induced in the presence of 9-cis RA (Figure 2B). Treatment of the cells with LG100268 equally resulted in induction of reporter gene expression. In contrast, treatment with LG100754 had no effect, in agreement with the in vitro binding assay. Despite the high expression levels of SRC1 in these cells (data not shown), coexpression of a dominant-negative form of SRC1 (SRCdn; Kurokawa et al, 1995) reduced the RXR-dependent response (Figure 2C; 9-cis RA). The PPAR:RXR-dependent response, on the other hand, is not or only weakly altered by SRCdn (Figure 2C; Wy and BRL, respectively). Altogether, the results show that RXR homodimer antagonists inhibit the formation of RXR:SRC1 ternary complexes. In contrast, RXR:RXR:SRC1 complexes are induced by RXR agonists and are able to mediate reporter gene induction when transfected into exogenous cells. Figure 2.RXR antagonists preclude RXR:RXR/SRC-1 complex formation. (A) EMSA: Radiolabelled MEp (left panel) or the synthetic RXRE DR1G (right panel) was incubated with RXRα and SRC1(B) in the presence or absence of 1 μM 9-cis RA, LG100268 or LG100754 as indicated. The open arrowhead indicates the RXR homodimer complex, and the black arrowhead indicates the ternary complex. (B) NIH-3T3 cells were transfected with the MEp-containing reporter gene, RXR encoding expression vector, and pCMV-β-galactosidase as internal control for transfection efficiency. Following transfection, the cells were cultivated for 48 h in the presence of 1 μM 9-cis RA, LG100268 or LG100754 as indicated, or with vehicle alone. CAT activity levels were standardized for β-galactosidase expression and expressed as fold induction with respect to the CAT activity obtained in the presence of the vehicle alone. (C) NIH-3T3 cells were transfected with the MEp-containing reporter gene and either an RXR- or a PPAR-encoding expression vector as indicated, together with pCMV-β-galactosidase as internal control for transfection efficiency. A vector expressing a dominant-negative form of SRC1 (SRCdn) was added at increasing doses, as indicated; total transfected DNA was maintained equal under each condition by use of the corresponding empty vector. Numbers indicate the relative proportions (μg) of transfected expression vectors. Following transfection, the cells were cultivated for 48 h in the absence or presence of either 1 μM 9-cis RA, 100 μM Wy14,643, 5 μM BRL49653 or vehicle alone. CAT activity levels were standardized for β-galactosidase expression and expressed as fold induction with respect to the activity observed in the presence of vehicle alone. Download figure Download PowerPoint We next analysed the specificity of RXR homodimer/coactivator interaction by performing EMSA in the presence of the nuclear receptor interaction domains of TIF2 and p300. Similar to our observations with SRC1, TIF2 is able to stabilize the binding of 9-cis RA-activated RXR homodimers to MEp (Figure 3). In contrast, p300 is unable to interact with the DNA-bound RXR homodimer, but readily forms a complex with PPAR:RXR regardless of the presence of ligand. As p300 has been shown to interact with RXR in solution, this latter result further suggests an allosteric role for DNA, which affects nuclear receptor/coactivator interactions. Figure 3.Specificity of RXR/coactivator association. EMSA: Radiolabelled MEp was incubated with PPARγ and RXRα (A), or RXR alone (B) and the receptor interacting domains of either SRC1 (amino acids 1226–1441; S), TIF2 (amino acids 624–869; T) or p300 (amino acids 31–220; 300), in the presence or absence of 1 μM 9-cis RA or 5 μM BRL49643 as indicated. The open arrowhead indicates the RXR homodimer, and the black arrowhead indicates the ternary complex. Download figure Download PowerPoint These results emphasize that, in vitro, RXR homodimer formation onto PPREs requires selective and ligand-dependent interaction with specific coactivators. We next addressed the in vivo relevance of this RXR homodimer signalling mechanism. To this end, we analysed the regulation of well-characterized PPAR target genes in vivo, in the presence and absence of PPARs (Figure 4A). By crossing PPARα−/− and PPARβ−/− mice (Peters et al, 1997; Tan et al, 2001), we obtained PPARα−/−β−/− pups from which we derived primary keratinocyte cultures. These cells can be considered as devoid of PPARs since PPARγ is undetectable in keratinocytes (Tan et al, 2001). As seen in Figure 4B, the PPRE reporter gene transfected in wild-type primary keratinocytes is activated to a similar extent by the PPAR ligand and by 9-cis RA. In PPARα−/−β−/− keratinocytes, regulation by 9-cis RA remains unaffected, whereas regulation by the PPAR ligand is lost. Finally, regulation by both ligands is lost in cells derived from pups with a keratinocyte-specific deletion of RXRα (Li et al, 2001). Subsequent ChIP analysis performed on the corresponding cell extracts demonstrates that in wild-type cells, both PPAR and RXR are able to bind to the transfected PPRE in the presence of either RXR or PPAR activator (Figure 4C). Importantly, and as proof of the concept, 9-cis RA still induces RXR binding to the reporter gene PPRE in the absence of PPAR. Using the same approach, identical complex formation is observed onto the endogenous PPRE of the PDK1 gene (Figure 4C), a newly characterized PPAR target gene (Di Poï et al, 2002). These results demonstrate that in vivo RXR homodimers can bind to functional PPREs, both endogenous and transfected elements, and mediate transactivation. Figure 4.In vivo cofactor-specific binding of RXR homodimers to PPREs. Experiments were performed using primary keratinocyte cultures derived from either wild-type mice (WT), compound knock-out mice for PPARα and PPARβ (PPARα−/−β−/−), and mice carrying a keratinocyte-specific deletion of RXRα (RXRα−/−). Primary cultures were transfected with a PPRE-luciferase reporter gene and treated with either PPAR ligands (P; a mixture of 10 μM Wy14,643 and 5 μM L1165041), RXR ligand (X; 0.5 μM 9-cis RA) or vehicle (V; DMSO), and subjected to analyses. (A) Schematic outline of the procedure. (B) Reporter gene induction in response to various ligands. Luciferase activity levels were standardized for β-galactosidase expression used as internal control of transfection efficiency, and expressed as the fold induction with respect to the activity observed with the control reporter gene. (C) PPAR and RXR binding to PPREs in vivo. Chromatin from the various primary keratinocyte cultures was immunoprecipitated with either a pan-PPAR antibody (PPAR-Ab), an RXR antibody (RXR-Ab) or preimmune serum (p.i.). Enrichment of either a DNA fragment encompassing the PPRE from the transfected reporter gene (rep PPRE), a PPRE-containing fragment from the endogenous PDK1 promoter (PDK1 PPRE) or a control DNA fragment from the PDK1 gene (PDK1 control) was evaluated by PCR. Aliquots of the extracts were also analysed before immunoprecipitation (input). (D) Ligand-dependent coactivator association in solution. Western blot analyses were performed on proteins after immunoprecipitation (IP) with either an antibody against PPAR (PPAR-Ab) or RXR (RXR-Ab). Proteins were separated by SDS–PAGE, blotted and probed with either SRC1 or TIF2 primary antibody as indicated. Bands were detected by chemiluminescence with horseradish peroxidase. The Western blot control with tubulin antibody was performed on cell lysates prior to immunoprecipitation. (E) SRC1 binds to DNA-bound RXR, in a 9-cis RA-dependent manner. ChIP of PPRE-containing genes was performed as described in (C), using anti-SRC1 or preimmune serum (p.i.). Download figure Download PowerPoint We next examined RXR homodimer interaction with coactivators in vivo. To address this, we first performed Western blot analysis to evaluate the interaction between nuclear receptor and cofactor, either on DNA or in solution. Immunoprecipitation performed with an RXR antibody revealed that both SRC1 and TIF2 strongly interact with RXR in the presence of 9-cis RA (Figure 4D). Fainter but clearly positive signals are also seen in the extracts obtained with the PPAR antibody. To discriminate between interaction in solution and when bound to DNA, we next performed a ChIP using an SRC1 antibody. The results demonstrate that ligand-activated PPAR loses its ability to interact with SRC1 upon binding to the response element (compare Figure 4D and E). In contrast, SRC1 is specifically recruited to the PPRE by activated RXR, irrespective of the presence of PPAR (Figure 4E). These results show that in the absence of PPAR, RXR homodimers can functionally substitute for PPARs by binding to PPREs as a complex with SRC1. It can be argued that the homodimer pathway uncovered herein may be an artifact of deleting PPARs. Therefore, to support the existence of a functionally relevant RXR homodimer pathway even in the presence of PPARs, we performed a sequential ChIP experiment using primary keratinocyte cultures derived from wild-type mice. Following treatment with 9-cis RA, we performed a first ChIP using either an SRC1 or a p300 antibody. Subsequently, the antibodies were dissociated from the complexes, and a second ChIP was performed with either a PPAR or an RXR antibody (Figure 5). If SRC1 is exclusively recruited by activated RXR homodimers, PCR amplification of the PDK1 gene should only be observed following sequential immunoprecipitation with SRC1 and RXR antibodies. Indeed, no amplification is observed after the second immunoprecipitation with the PPAR antibody, showing that the complex pulled down with the SRC1 antibody contains RXR but no PPAR (Figure 5A, compare lanes 3 and 4). In contrast, and in agreement with our results from Figure 3, the complex pulled down with the p300 antibody contains both PPAR and RXR (Figure 5A, lanes 6 and 7). The same results were obtained using BMS 649, a synthetic RXR specific ligand (Egea et al, 2002) (Figure 5B). This clearly shows that RXR homodimer signalling via PPREs occurs in vivo and requires SRC1, whereas DNA-bound PPAR:RXR does not interact with SRC1. To further demonstrate the functional significance of SRC1 for RXR homodimer signalling, primary keratinocytes from wild-type and SRC1−/− mice (Xu et al, 1998) were transfected with a PPRE reporter gene and treated with either PPAR- or RXR-specific ligands (Figure 5C). The RXR agonist-induced response in SRC1−/− cells was significantly reduced as compared to wild-type cells, whereas PPAR agonist-induced reporter gene activity remained unaffected. Thus, in the presence of DNA, coactivators constitute a molecular switch between two competing signalling pathways, PPAR:RXR and RXR homodimers. Figure 5.RXR:RXR/SRC1 ternary complexes bind to PPREs in the presence of PPAR. Primary keratinocytes from wild-type mice were treated with either 0.5 μM 9-cis RA (A), 1 μM BMS 649 (B) or vehicle. Following immunoprecipitation (ChIP) with either an SRC1-specific antibody (SRC1), a p300-specific antibody (p300) or preimmune serum (p.i.), the complexes were subjected to a second immunoprecipitation (re-ChIP) with either a PPAR- (P) or an RXR- (R) specific antibody. Enrichment of a PPRE-containing fragment from the endogenous PDK1 promoter (PDK1 PPRE) and a control DNA fragment from the PDK1 gene (PDK1 control) was evaluated by PCR. Aliquots of the extracts were also analysed before immunoprecipitation (input). (C) Primary keratinocytes from either wild-type (grey bars) or SRC1−/− (hatched bars) mice were transfected with a PPRE-luciferase reporter gene and treated with either vehicle (V; DMSO), PPAR ligands (P; a mixture of 10 μM Wy14,643 and 5 μM L1165041) or increasing doses of BMS as indicated. Luciferase activity levels were standardized for β-galactosidase expression used as internal control of transfection efficiency and expressed as the fold induction with respect to the activity observed with the activity of the reporter gene in the presence of vehicle alone. Download figure Download PowerPoint The malic enzyme (ME) gene used throughout this study is abundantly expressed in the liver and is induced by both PPAR and RXR activators. Only low levels of SRC1 are expressed in the liver; however, TIF2 is abundantly expressed and interacts with both PPAR and RXR, at least in solution (Figure 6A and B). To further demonstrate the relevance of the RXR homodimer pathway in vivo, wild-type and PPARα−/− mice were treated for 5 days either with the PPARα ligand Wy14,643, or 9-cis RA. Analysis by ChIP of liver extracts from wild-type mice shows that RXR binds to MEp in the presence of either activator. In the absence of PPARα, RXR no longer binds to the PPRE upon treatment with the PPARα activator. However, in the presence of 9-cis RA, RXR still binds the PPRE and is able to induce the activity of the ME gene (Figure 6C, left and middle panels, and Figure 6D). Remarkably, ChIP using a TIF2 antibody results in an MEp
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