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Cryo-electron tomography: The challenge of doing structural biology in situ

2013; Rockefeller University Press; Volume: 202; Issue: 3 Linguagem: Inglês

10.1083/jcb.201304193

1540-8140

Vladan Lučić, Alexander Rigort, Wolfgang Baumeister,

Photosynthetic Processes and Mechanisms

Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technical advances include sample vitrification that faithfully preserves molecular structures, three-dimensional imaging by electron tomography, and improved image-processing methods. These new techniques have enabled the extraction of high fidelity structural information and are beginning to reveal the macromolecular organization of unperturbed cellular environments.