VWF and ADAMTS13 behavior in estradiol-treated HUVEC
2010; Wiley; Volume: 86; Issue: 2 Linguagem: Inglês
10.1111/j.1600-0609.2010.01545.x
ISSN1600-0609
AutoresYanina Powazniak, Ana Catalina Kempfer, Julio César Calderazzo Pereyra, Juvenal Paiva, M.A. Lazzari,
Tópico(s)Complement system in diseases
ResumoEuropean Journal of HaematologyVolume 86, Issue 2 p. 140-147 VWF and ADAMTS13 behavior in estradiol-treated HUVEC Yanina Powazniak, Yanina Powazniak FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorAna Catalina Kempfer, Ana Catalina Kempfer FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorJulio César Calderazzo Pereyra, Julio César Calderazzo Pereyra FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorJuvenal Paiva Palomino, Juvenal Paiva Palomino FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorMaria Angela Lazzari, Maria Angela Lazzari FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this author Yanina Powazniak, Yanina Powazniak FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorAna Catalina Kempfer, Ana Catalina Kempfer FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorJulio César Calderazzo Pereyra, Julio César Calderazzo Pereyra FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorJuvenal Paiva Palomino, Juvenal Paiva Palomino FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this authorMaria Angela Lazzari, Maria Angela Lazzari FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, ArgentinaSearch for more papers by this author First published: 19 October 2010 https://doi.org/10.1111/j.1600-0609.2010.01545.xCitations: 8 Yanina Powazniak, FONCyT/CONICET, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Pacheco de Melo 3081 (C1425AUM), Buenos Aires, Argentina. Tel: 0541148055759; Fax: 0541148050712; e-mail: yaninapowazniak@gmail.com Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Objectives: In this study, the role of 17β-estradiol (E2) in the regulation of von Willebrand factor (VWF) and ADAMTS13 synthesis, storage, and secretion was investigated in cultured human umbilical vein endothelial cells (HUVEC). Methods: HUVEC were grown to 80–90% confluence and replaced with fresh medium containing E2 (1 nm) or vehicle for 24 h, after which the supernatant medium and cell lysates were collected to measure VWF and ADAMTS13. VWF was evaluated by VWF:Ag and multimeric analysis. ADAMTS13 was evaluated by SDS-PAGE. VWF and ADAMTS13 mRNA were quantified by real-time PCR after E2 or vehicle exposure for 18 h. A functional effect of ADAMTS13 on HUVEC VWF protein synthesis was further evaluated using a short hairpin RNA (shRNA) to knockdown the expression of endogenous ADAMTS13. Results: E2 did not increase the release or intracellular VWF levels in HUVEC. However, E2 increased the production of intracellular ADAMTS13, although there was no evidence of significant effects of their release into culture medium. Incubation of HUVEC with E2 resulted in a significantly increased expression of VWF and ADAMTS13 mRNA. ADAMTS13 gene inactivation upregulates release and intracellular VWF levels in E2-treated HUVEC. Conclusion: The results demonstrated that E2 may play a role in the regulation of VWF and ADAMTS13 gene expression and in its production in human endothelial cells. The mechanism of the protective effects of E2 on the cardiovascular system could be explained by the intracellular regulation of VWF produced by ADAMTS13. Citing Literature Volume86, Issue2February 2011Pages 140-147 RelatedInformation
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