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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis

2011; BioMed Central; Volume: 9; Issue: 1 Linguagem: Inglês

10.1186/1479-0556-9-5

ISSN

1479-0556

Autores

Luciana Previato de Almeida, A. P. F. Trombone, Júlio CC Lorenzi, Carolina D. Rocha, Thiago Malardo, Isabela C Fontoura, Ana Flávia Gembre, Ricardo LL Silva, Célio Lopes Silva, Ademílson Panunto-Castelo, Arlete Aparecida Martins Coelho‐Castelo,

Tópico(s)

Hepatitis B Virus Studies

Resumo

Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown.In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge.In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice.These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.

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