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The Inhibition of Clostridium chauvoei (Jakari strain) Neuraminidase Activity by Methanolic Extracts of the Stem Barks of Tamarindus indicus and Combretum fragrans

2004; Taylor & Francis; Volume: 19; Issue: 4 Linguagem: Inglês

10.1080/14756360409162447

1475-6374

N. M. Useh, Andrew J. Nok, Suleiman Folorunsho Ambali, K.A.N. Esievo,

Medicinal Plants and Neuroprotection

The inhibition of neuraminidase from Clostridium chauvoei (jakari strain) with partially purified methanolic extracts of some plants used in Ethnopharmacological practice was evaluated. Extracts of two medicinal plants, Tamarindus indicus and Combretum fragrans at 100–1000 μg/ml, both significantly reduced the activity of the enzyme in a dose-dependent fashion (P < 0.001).The estimated IC50 values for Tamarindus indicus and Combretum fragrans were 100 and 150 μ/ml respectively. Initial velocity studies conducted, using fetuin as substrate revealed a non-competitive inhibition with the Vmax significantly altered from 500 μmole min−1 mg−1 to 240μmole min−1 mg−1 and 340 μmole min−1 mg−1 in the presence of Tamarindus indicus and Combretum fragrans respectively. The KM remained unchanged at 0.42 mM. The computed Index of physiological efficiency was reduced from 1.19 min−1 to 0.57 min−1 and 0.75 min−1 with Tamarindus indicus and Combretum fragrans as inhibitors respectively.