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Immortalization of bone marrow-derived porcine mesenchymal stem cells and their differentiation into cells expressing cardiac phenotypic markers

2011; Wiley; Volume: 6; Issue: 8 Linguagem: Inglês

10.1002/term.469

1932-7005

Isabel Moscoso, José-Ignacio Rodríguez-Barbosa, Javier Barallobre‐Barreiro, P. Añón, Nieves Doménech,

Electrospun Nanofibers in Biomedical Applications

Journal of Tissue Engineering and Regenerative MedicineVolume 6, Issue 8 p. 655-665 Research Article Immortalization of bone marrow-derived porcine mesenchymal stem cells and their differentiation into cells expressing cardiac phenotypic markers Isabel Moscoso, Isabel Moscoso Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorJose-Ignacio Rodriguez-Barbosa, Jose-Ignacio Rodriguez-Barbosa Laboratorio de Inmunobiología, Instituto de Biomedicina, Campus de Vegazana s/n, 24071 Leon, SpainSearch for more papers by this authorJavier Barallobre-Barreiro, Javier Barallobre-Barreiro Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorPatricia Anon, Patricia Anon Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorNieves Domenech, Corresponding Author Nieves Domenech [email protected] Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainN. Domenech, Unidad de Investigacion, Complejo Universitario Universitario A Coruña, Xubias de Arriba 84, 15006-A Coruña, Spain. E-mail: [email protected]Search for more papers by this author Isabel Moscoso, Isabel Moscoso Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorJose-Ignacio Rodriguez-Barbosa, Jose-Ignacio Rodriguez-Barbosa Laboratorio de Inmunobiología, Instituto de Biomedicina, Campus de Vegazana s/n, 24071 Leon, SpainSearch for more papers by this authorJavier Barallobre-Barreiro, Javier Barallobre-Barreiro Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorPatricia Anon, Patricia Anon Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainSearch for more papers by this authorNieves Domenech, Corresponding Author Nieves Domenech [email protected] Unidad de Investigación, INIBIC-Complejo Universitario Universitario A Coruña, SpainN. Domenech, Unidad de Investigacion, Complejo Universitario Universitario A Coruña, Xubias de Arriba 84, 15006-A Coruña, Spain. E-mail: [email protected]Search for more papers by this author First published: 09 December 2011 https://doi.org/10.1002/term.469Citations: 5Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Mesenchymal stem cells (MSCs) may be among the first stem cell types to be utilized in the clinic for cell therapy, because of their ease of isolation and extensive differentiation potential. Using a porcine model, we have established several cell lines from MSCs to facilitate in vitro and in vivo studies of their potential use for cellular therapy. Bone marrow-derived primary MSCs were immortalized using the pRNS-1 plasmid. We obtained four stable immortalized cell lines that exhibited higher proliferative capacities than the parental cells. All four cell lines displayed a common phenotype similar to that of primary mesenchymal cells, characterized by constitutively high expressions of CD90, CD29, CD44, SLA I and CD46, while CD172a, CD106 and CD56 were less expressed. Remarkably, treatment with 5-azacytidine-stimulated porcine MSCs lines to differentiate into cells that were positive for cardiac phenotypic markers, such as α-actin, connexin-43, sarcomeric actin, serca-2 and, to a lesser extent, desmin and troponin-T. These porcine MSC lines will be valuable biological tools for developing strategies for ex vivo expansion and differentiation of MSCs into a specific lineage. Copyright © 2011 John Wiley & Sons, Ltd. Citing Literature Volume6, Issue8August 2012Pages 655-665 RelatedInformation