Identification of Hepatitis C Virus Transmission Using a Next-Generation Sequencing Approach
2012; American Society for Microbiology; Volume: 50; Issue: 4 Linguagem: Inglês
10.1128/jcm.00005-12
ISSN1098-660X
AutoresAlejandro Escobar‐Gutiérrez, Mauricio Vázquez-Pichardo, Mayra Cruz‐Rivera, Pilar Rivera-Osorio, Juan Carlos Carpio-Pedroza, Juan Alberto Ruiz‐Pacheco, Karina Ruiz‐Tovar, Gilberto Vaughan,
Tópico(s)HIV/AIDS drug development and treatment
ResumoHere, we describe a transmission event of hepatitis C virus (HCV) among injection drug users. Next-generation sequencing (NGS) was used to assess the intrahost viral genetic variation. Deep amplicon sequencing of HCV hypervariable region 1 allowed for a detailed analysis of the structure of the viral population. Establishment of the genetic relatedness between cases was accomplished by phylogenetic analysis. NGS is a powerful tool with applications in molecular epidemiology studies and outbreak investigations.
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