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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

2011; BioMed Central; Volume: 12; Issue: 1 Linguagem: Inglês

10.1186/gb-2011-12-1-r1

1474-760X

Sheila Fisher, Andrew J. Barry, Justin Abreu, Brian Minie, Jillian Nolan, Toni Delorey, Geneva Young, Timothy J. Fennell, Alexander G. Allen, Lauren Ambrogio, Aaron M. Berlin, Brendan Blumenstiel, Kristian Cibulskis, Dennis C. Friedrich, Ryan C. Johnson, Frank Juhn, Brian Reilly, Ramy Shammas, John Stalker, Sean M. Sykes, J. E. Thompson, John B. Walsh, Andrew Zimmer, Zac Zwirko, Stacey B. Gabriel, Robert Nicol, Chad Nusbaum,

Molecular Biology Techniques and Applications

Abstract Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.