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Self‐Immobilizing Fluorogenic Imaging Agents of Enzyme Activity

2010; Wiley; Volume: 50; Issue: 1 Linguagem: Inglês

10.1002/anie.201005705

1521-3773

David H. Kwan, Hong‐Ming Chen, Khakhanang Ratananikom, Susan M. Hancock, Yoichiro Watanabe, Prachumporn T. Kongsaeree, Lacey Samuels, Stephen G. Withers,

Cancer Research and Treatments

Lighting up enzyme activity: Fluorogenic enzyme substrates have been developed that upon hydrolysis form reactive quinone methides, which can react with cell components in vivo to generate an immobilized fluorescent label (see scheme). These compounds can be used for the histological staining of tissues or activity-based screening of cells by fluorescence-activated cell sorting. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.