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A simple method for DNA isolation from Xanthomonas spp.

2000; UNIVERSIDADE DE SÃO PAULO; Volume: 57; Issue: 3 Linguagem: Inglês

10.1590/s0103-90162000000300028

1678-992X

Luiz Humberto Gomes, Keila Maria Roncato Duarte, Felipe Gabriel Andrino, Flávio César Almeida Tavares,

Legume Nitrogen Fixing Symbiosis

A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.