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Getting to the heart of the matter: long non-coding RNAs in cardiac development and disease

2013; Springer Nature; Volume: 32; Issue: 13 Linguagem: Inglês

10.1038/emboj.2013.134

1460-2075

Johanna C. Scheuermann, Laurie A. Boyer,

MicroRNA in disease regulation

Review11 June 2013free access Getting to the heart of the matter: long non-coding RNAs in cardiac development and disease Johanna C Scheuermann Johanna C Scheuermann Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA Search for more papers by this author Laurie A Boyer Corresponding Author Laurie A Boyer Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA Search for more papers by this author Johanna C Scheuermann Johanna C Scheuermann Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA Search for more papers by this author Laurie A Boyer Corresponding Author Laurie A Boyer Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA Search for more papers by this author Author Information Johanna C Scheuermann1,‡ and Laurie A Boyer 1,‡ 1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA ‡These authors contributed equally to this work. *Corresponding author. Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Tel.:+1 617 324 3335; Fax:+1 617 253 8699; E-mail: [email protected] The EMBO Journal (2013)32:1805-1816https://doi.org/10.1038/emboj.2013.134 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cardiogenesis in mammals requires exquisite control of gene expression and faulty regulation of transcriptional programs underpins congenital heart disease (CHD), the most common defect among live births. Similarly, many adult cardiac diseases involve transcriptional changes and sometimes have a developmental basis. Long non-coding RNAs (lncRNAs) are a novel class of transcripts that regulate cellular processes by controlling gene expression; however, detailed insights into their biological and mechanistic functions are only beginning to emerge. Here, we discuss recent findings suggesting that lncRNAs are important factors in regulation of mammalian cardiogenesis and in the pathogenesis of CHD as well as adult cardiac disease. We also outline potential methodological and conceptual considerations for future studies of lncRNAs in the heart and other contexts. Introduction The human genome project has opened the door for understanding development and disease at an unprecedented level. Once referred to as dark matter or 'junk DNA', it is now thought that up to 90% of the human genome is actively transcribed and produces many different types of transcripts including protein-coding and non-coding RNA (Carninci et al, 2005; Kapranov et al, 2007; ENCODE Project Consortium et al, 2012). Non-coding RNAs have been broadly classified according to transcript length as small and long non-coding RNAs (lncRNAs), where lncRNAs are considered to be greater than 200 nucleotides and can comprise up to thousands of nucleotides. The biological and mechanistic functions of different small non-coding RNA species have been extensively reviewed (Matera et al, 2007; Carthew and Sontheimer, 2009; Ghildiyal and Zamore, 2009; Malone and Hannon, 2009; Castel and Martienssen, 2013). Much less is known, however, about the functions of lncRNAs, an apparently heterogeneous class of RNA molecules with emerging biological functions. LncRNAs are pervasively transcribed throughout the genome and the resulting transcripts display remarkable similarities to classical mRNAs in that they are transcribed by RNA Polymerase II (RNAP2) and are generally, but not always, alternatively spliced, 5′-capped, and polyadenylated (Derrien et al, 2012). In contrast to protein-coding genes, lncRNAs have limited coding potential as indicated by the lack of protein domains or significant open reading frames (ORFs). Moreover, lncRNAs display random codon usage and no significant bias towards silent nucleotide substitutions underscoring their low protein-coding potential; and these transcripts are rarely translated despite their engagement with ribosomes in some cases (Cabili et al, 2011; Lin et al, 2011; Ulitsky et al, 2011; Bánfai et al, 2012). Based largely on these criteria, a large number of lncRNA have been identified across eukaryotes, thousands of them in mouse and human (Ulitsky et al, 2011; Derrien et al, 2012). Remarkably, lncRNAs appear to be rapidly evolving and generally display low levels of sequence conservation. About one-third of all human lncRNAs have arisen only within the primate lineage (Derrien et al, 2012), while only a small subgroup of lncRNAs appear to be maintained throughout a range of species with conservation being generally most evident in their promoter regions (Chodroff et al, 2010; Ulitsky et al, 2011). Together, these data suggest that regulation of lncRNA expression patterns is important for the function of this class of transcripts. Based on their genomic location, lncRNAs can be further grouped into different classes of transcripts (Ulitsky et al, 2011; Derrien et al, 2012) (Figure 1A). For example, long intergenic (or intervening) non-coding RNAs (lincRNAs) are located between coding or non-coding genes, and do not overlap the exons of other genes. lncRNA loci can also reside within the introns of protein-coding genes. Natural antisense transcripts (NATs) are produced from the opposite strand of a coding (or non-coding gene) but their transcription start site is located downstream relative to that of the coding gene, and these transcripts often display at least partial overlap with the coding sequence of the corresponding mRNA. LncRNAs are highly versatile in that they can partially base pair with other RNA templates to form duplexes or with DNA which can lead to the formation of triplex structures (Figure 1B). Moreover, these transcripts can interact with a diverse repertoire of proteins. Thus, lncRNAs are thought to possess tremendous regulatory potential. Figure 1.LncRNAs are a heterogeneous class of transcripts and function to regulate gene expression by diverse mechanisms. (A) Representative classes of long non-coding RNAs based on genomic location. LncRNAs can be located and transcribed within introns of protein-coding genes (left), as intervening genes known as long intergenic or intervening non-coding RNAs (lincRNAs) that do not overlap with the exons of other genes (middle), or they can be located on the opposite strand of a coding or non-coding gene and transcribed in the antisense direction (right). (B) Global mechanisms of lncRNA function. LncRNAs can function as molecular scaffolds by interacting with proteins such as transcription factors or components of chromatin modifying complexes to affect positive or negative regulation of gene expression (left panel). Proposed mechanisms of action include targeting proteins to specific genomic sites such as promoter regions by complementary interactions with DNA. Alternatively, interactions with DNA may prevent the binding of specific factors to the DNA template (middle). LncRNAs can also base pair with other RNA molecules such as mRNAs or may act as a sponge for miRNAs. This scenario is thought to lead to post-transcriptional gene silencing (right panel). Download figure Download PowerPoint In general, lncRNAs can act in cis to regulate neighbouring genes or in trans to modulate the expression of their target genes by employing a wide range of molecular mechanisms. For example, a subclass of lncRNAs with apparent enhancer-like activity, termed as ncRNA-activating (ncRNA-a), has recently been found to activate neighbouring genes in cis using a mechanism involving DNA looping between the lncRNA and its target gene (Orom et al, 2010; Lai et al, 2013). Evidence also indicates that a subset of lncRNAs regulates gene expression by acting in trans as recruiters or decoys for chromatin modifiers and transcription factors to activate or silence genes (Rinn et al, 2007; Khalil et al, 2009; Tsai et al, 2010; Ng et al, 2012; Grote et al, 2013; Klattenhoff et al, 2013). Notably, despite the lack of sequence conservation among lncRNAs, interactions with RNA binding proteins including Polycomb and Trithorax group members have been widely conserved between mouse and human (Guttman and Rinn, 2012). Alternatively, some lncRNAs have been reported to function as microRNA sponges, titrating these small transcripts away from their respective mRNA target (Cesana et al, 2011; Wang et al, 2013). LncRNAs have also been reported to influence mRNA splicing, translation, or degradation by binding to mRNAs or protein components of RNP complexes (Tripathi et al, 2010; Gong and Maquat, 2011; Yoon et al, 2012). Thus, lncRNAs have roles in transcriptional and post-transcriptional gene regulatory events. Consequently, the biological functions of lncRNAs as well as their mechanisms of action are expected to be diverse and will require a great deal of functional subclassification in the future. While most predicted lncRNAs await functional characterization, there are clear examples demonstrating prominent roles for these transcripts in a variety of cellular processes including dosage compensation (e.g., X chromosome inactivation), imprinting, regulation of cell cycle, and apoptosis (Rinn and Chang, 2012; Lee and Bartolomei, 2013). Moreover, lncRNAs have been shown to play roles in somatic cell differentiation programs as well as maintenance of cell fate (Guttman et al, 2011; Hu et al, 2011; Kretz et al, 2012, 2013). Thus, lncRNAs may represent a new layer of regulation in differentiation and lineage commitment. Consistent with these broad roles, this class of transcripts has also been implicated as contributing factors to diseases with developmental components such as cancer and neurological disorders (Ponting et al, 2009; Wapinski and Chang, 2011; Batista and Chang, 2013; Mercer and Mattick, 2013; Ng et al, 2013). The emerging links between lncRNAs and disease as well as their tissue-specific expression patterns indicate that lncRNAs comprise a core transcriptional regulatory circuitry with master regulators and further suggest that they represent new molecules for targeted therapy. Mammalian heart development is a tightly regulated process requiring exquisite control of transcriptional programs. Consistent with this idea, disruption of transcriptional networks underpins congenital heart disease (CHD) and certain forms of adult cardiac disease (Srivastava, 2006a; Bruneau, 2008). In fact, heart disease is the leading cause of morbidity and mortality worldwide (WHO 2011) with dramatic effects on the life quality of patients as well as on the health-care system. Thus, dissecting the transcriptional regulatory principles that govern heart development and tissue homeostasis in the adult heart is of great interest to developmental and molecular biologists as well as to clinicians. While it is known that the activities of DNA binding transcription factors, chromatin regulators, and signalling molecules converge to control tissue-specific gene expression programs during heart development (Olson, 2006; Srivastava, 2006b; Chang and Bruneau, 2012; Bruneau, 2013), it is likely that non-coding transcripts also contribute to this highly orchestrated process. For example, members of the class of small non-coding RNAs such as miRNAs have critical roles in fine-tuning gene expression patterns during heart development (Cordes and Srivastava, 2009; Liu and Olson, 2010). Of particular interest is the recent discovery of lncRNAs that function in cardiac lineage commitment and heart development, revealing an additional layer of complexity (Grote et al, 2013; Klattenhoff et al, 2013). Although a general picture is emerging, we are only beginning to understand the implications of lncRNA regulation in heart development and cardiac-related disease. Here, we discuss the newly emerging roles of lncRNAs and cite-specific examples in the context of heart development and cardiac disease as well as present considerations for the identification of additional lncRNA regulators of this process from a methodological and conceptual point of view. Heart development is regulated by tight control of gene expression patterns Heart development requires the concurrent differentiation of multiple cell types that must organize into a complex structure. This process necessitates tight control of gene expression patterns in a temporal and spatial manner (Srivastava, 2006b). In most species, the primitive heart is established by concordant expression of a highly conserved core cardiac transcription factor network (Olson, 2006; McCulley and Black, 2012). However, more complex structures such as those that comprise the multichambered mammalian heart may require additional species-specific regulatory factors. For example, the Drosophila heart is shaped as a relatively simple linear tube consisting of three sections that transport the haemolymph along the anterior-posterior body axis: heart proper, posterior aorta, and anterior aorta, with the beating heart proper being separated from the aorta by a cardiovascular valve (Medioni et al, 2009; Seyres et al, 2012) (Figure 2A). Mammalian heart development begins with formation of an analogous structure in embryogenesis, yet the developing heart undergoes a series of complex movements, rotations, and subsequent refinement, resulting in a four-chambered heart with distinct in- and out-flow tracts, cardiac valves separating the different compartments, and a mature conduction system (Harvey, 2002; Srivastava, 2006b; Vincent and Buckingham, 2010) (Figure 2B). Figure 2.The developmental complexity of heart development varies among organisms despite conserved core cardiac TF network. (A) Drosophila heart development begins during embryonic stage 11 by specification of two contralateral rows of cardiogenic mesoderm and formation of cardioblasts. Cardioblasts migrate towards the midline at stage 13–14 and form a simple linear closed tube with a central lumen by stage 16–17, subsequently differentiating into more mature cardiomyocytes. (B) The first steps of mammalian heart development proceed in a very similar manner, yet the mature heart is considerably more complex with two atrial and two ventricular chambers, connecting the systemic and pulmonary circuits via four valves and in- and outgoing vessels. The earliest step of mammalian cardiogenesis involves the bilateral specification of cardiac progenitor cell populations from the first heart field (FHF) in the anterior lateral plate mesoderm, which condense into two lateral heart primordia (mouse E7.5, human day 15) to form the cardiac crescent. The secondary heart field (SHF) constitutes a separate cell population at the medial sides of the two processes of the cardiac crescent. The two processes of the cardiac crescent fuse to form a beating primitive linear heart tube (mouse E8.5, human day 21), which then undergoes rightward looping, resulting in formation of the early chambers (mouse E9, human day 28). During later stages, the mature shape of the heart is generated by differentiation of cardiac cell populations and extensive remodelling of the heart, resulting in four-chambered heart with distinct in- and outflow tracts, cardiac valves separating the different compartments, and a mature conduction system. (C) The core transcription factor network necessary for specification of the cardiovascular lineages is conserved between Drosophila and mammals. (D) The percentage of non-coding to protein-coding sequence increases with developmental complexity. Whereas S. cerevisiae dedicates most of its genome to protein-coding genes, only a small fraction of the genome codes for proteins in human. (E) The total number of putative lncRNA transcripts is predicted to be significantly higher in mouse (∼3000 lincRNA transcripts as determined by Ponjavic et al, 2007 and Sigova et al, 2013) and human (∼15 000 as defined by Derrien et al, 2012) as compared to lower eukaryotes such as Drosophila (17 based on stringent criteria in Tupy et al, 2005 to greater than 1000 based on low stringency estimates from Young et al, 2012), C. elegans (262 from Nam and Bartel, 2012), and zebrafish (∼700 transcripts predicted from Ultisky et al, 2011 and Pauli et al, 2012). The number of lncRNAs varies among studies as different criteria were used to define lncRNA transcripts. Download figure Download PowerPoint Despite the distinct structural differences between the simple Drosophila heart and the more complex mammalian heart, the core transcription factor network is highly conserved between both organisms (Reim and Frasch, 2010) (Figure 2C). In fact, Drosophila heart development has been used as a model to identify novel gene interactions leading to human heart disease (Qian et al, 2011; Qian and Bodmer, 2012). Mutations affecting cardiac transcription factors are often causative for congenital heart defects (Bruneau, 2008; McCulley and Black, 2012). For example, mutations in GATA4 or NKX2.5, members of the core cardiac transcription factor network, lead to atrial and ventricular septum defects and Tetralogy of Fallot. While the binding of sequence-specific transcription factors to gene regulatory elements such as promoters and distal enhancers drive heart development, it has become increasingly clear that additional mechanisms contribute to fine-tuning the cardiac regulatory network. For example, microRNAs (e.g., miR-1, -126, -138, -143, -145) have roles in cardiogenic processes including angiogenesis, establishment of cardiac cell polarity, development of the cardiac conduction system, cardiac patterning, or smooth muscle cell differentiation, respectively, and in many cases these miRNAs regulate and interact with the core cardiac transcriptional network (Cordes and Srivastava, 2009; Liu and Olson, 2010). Notably, some miRNAs have been shown to play analogous roles in Drosophila heart development (Nguyen and Frasch, 2006). Despite the similarities between lower eukaryotes such as Drosophila and more complex organisms, the mechanisms that give rise to the additional complexity of heart formation and function in mammals are not well understood. Given the recent identification of thousands of lncRNAs and their unique representation and tissue-specific expression across organisms, these transcripts may signify a new class of regulatory molecules in cardiac development, and their functions may represent key distinctions that give rise to the more complex substructures of the mammalian heart. LncRNAs function to regulate developmental transitions in cardiac commitment LncRNAs have emerged as potent regulators of gene expression and may represent an important part of what distinguishes higher organisms from simpler eukaryotes. While unicellular organisms dedicate the majority of their genome to protein-coding sequence (∼73% in S. cerevisiae), this fraction decreases significantly in multicellular organisms such as Drosophila (∼18%), and coding sequences represent only a minor fraction of DNA in mammals (e.g., ∼2–3% in humans) (Taft et al, 2007) (Figure 2D). Thus, it has been hypothesized that the ratio of non-coding compared to protein-coding transcripts in the genome, rather than the overall number of protein-coding genes, underpins organismal complexity. While this idea remains to be tested, the total number of lncRNAs transcripts increases dramatically from Drosophila to human (Figure 2E). For example, while mice and humans have thousands of putative lncRNA genes (Ponjavic et al, 2007; Ulitsky et al, 2011; Derrien et al, 2012; Sigova et al, 2013), recent estimates suggest that there are 17 (Tupy et al, 2005) and possibly upwards of ∼1000 lncRNA transcripts in Drosophila (Young et al, 2012), ∼170 loci in C. elegans that specify ∼272 lincRNAs (Nam and Bartel, 2012), and ∼600 loci that produce ∼700 transcripts in zebrafish (Ulitsky et al, 2011; Pauli et al, 2012). There are several caveats to this analysis, however, as numbers of putative lncRNAs or subclasses such as lincRNAs can vary widely among studies given that different criteria are used to identify these transcripts in each case. Furthermore, the transcriptomes of lower eukaryotes have not been as thoroughly analysed compared to mouse and human. Nevertheless, lncRNAs across multicellular organisms often display expression patterns that are highly tissue specific, suggesting that at least some of these transcripts have roles in developmental processes (Cabili et al, 2011; Derrien et al, 2012). For example, many lncRNAs are expressed dynamically at specific developmental stages during cardiomyocyte differentiation (Wamstad et al, 2012). Indeed, two recent studies identified lncRNAs in mouse with functions in commitment to the cardiac lineage and heart development (Klattenhoff et al, 2013; Grote et al, 2013), opening the door to the possibility that lncRNAs represent new modes of developmental regulation. Braveheart, an lncRNA required for the specification of a common cardiac progenitor The lncRNA Braveheart (Bvht, AK143260) was discovered in mouse based on its unique expression pattern. Bvht is expressed at early developmental stages in mouse embryonic stem cells (mESCs) and also abundantly in the adult heart relative to other differentiated tissues suggesting that this lncRNA may be important for specification of the cardiac lineage. Consistent with this idea, depletion of Bvht in mESCs impaired formation of cardiomyocytes in multiple in vitro differentiation assays (Klattenhoff et al, 2013). Heart development involves the specification of cardiac progenitor cells within the lateral plate mesoderm. MESP1, an essential transcription factor that is conserved in vertebrates and some non-vertebrate chordate species (Saga et al, 1996, 1999, 2000, 2000; Satou et al, 2004; Kriegmair et al, 2013), marks the earliest known cardiac population during development as well as tissues that contribute to head mesenchyme (Bondue et al, 2008; David et al, 2008; Lindsley et al, 2008). MESP1 progenitors have the capacity to specify all cell types of the heart, including cardiomyocytes, endothelial cells, and cardiac smooth muscle cells. Notably, using an in vitro cardiomyocyte differentiation system, the investigators found that Bvht is required for induction of MesP1 and its downstream targets including the core cardiac transcription factors Gata4, Gata6, Hand1, Hand2, Tbx2, and Nkx2.5, among others. In contrast, mesoderm markers such as Brachyury and Eomes were expressed normally at early stages of differentiation and remained expressed upon loss of Bvht. Notably, both BRACHYURY and EOMES are necessary for proper induction of MesP1 (Costello et al, 2011; David et al, 2011). Together, these data suggested that Bvht is necessary for the transition from nascent to cardiac mesoderm. LncRNAs can function in trans by interacting with chromatin modifiers to mediate changes in gene expression (Guttman and Rinn, 2012). Notably, Bvht was found to interact with SUZ12, a core component of the Polycomb repressive complex 2 (PRC2) and loss of the lncRNA resulted in a failure to activate the cardiac gene expression program. Many of the transcription factors in the core cardiac network are targets of PRC2 mediated repression in ESCs and differentiation towards specific lineages requires the selective loss of PRC2 at subsets of these genes (Boyer et al, 2006). Upon Bvht depletion, PRC2 and its associated repressive modification H3K27me3 remained enriched at the promoters of critical genes in the cardiovascular network, including MesP1, Gata6, Hand1, Hand2, and Nkx2.5. Thus, Bvht may function as a molecular decoy to regulate expression of the core cardiac network (Figure 3A). However, whether Bvht regulates expression of the core cardiac network directly via its PRC2 binding activity or if it employs additional molecular mechanisms to promote cardiac commitment remains an open question. Figure 3.Mechanisms of lncRNA function in heart development and cardiac disease. (A) Braveheart is necessary for commitment to the cardiac lineage in mouse. Bvht appears to function in trans through interaction with the epigenetic silencing complex PRC2 and may act as a decoy to antagonize its recruitment to key developmental genes during cardiomyocyte differentiation. Alternatively, Bvht may recruit PRC2 to gene(s) that repress the cardiac program. In either case, loss of Bvht leads to a failure to activate the core cardiac gene network that includes many TFs implicated in heart development and disease. (B) Fendrr is expressed in the lateral plate mesoderm in mouse from which precursors for the heart and body wall are derived. Fendrr is proposed to function partly in cis to regulate its neighbouring gene Foxf1a. Fendrr also functions in trans to regulate the expression of additional genes important for heart development. Fendrr interacted with PRC2 components as well as WDR5, a member of TrxG/MLL complex suggesting that Fendrr regulates the balance between repressive and activating marks at key genes during development. Thus, Bvht and Fendrr may represent examples of lncRNAs that regulate gene expression through epigenetic mechanisms. (C–E) LncRNAs can also function as natural antisense transcripts (NATs) to affect gene expression at the transcriptional and post-transcriptional level. ANRIL was identified as a risk factor for coronary disease by GWAS. ANRIL is expressed in the opposite direction to INK4B/P15 in the INK4 locus. The antisense transcript appears to recruit PRC1 and PRC2 to mediate repression of the INK4a/INK4b tumour suppressor locus through an epigenetic silencing mechanism (C). The ratio of two important sarcomere components MYH6 and MYH7 vary during development and in stress-induced pathological conditions. Myh6 and Myh7 genes are juxtaposed in the mouse genome in a head-to-tail fashion. An antisense lncRNA (Myh7-as) is transcribed across the Myh7 locus and negatively correlates with MYH7 abundance. Thus, Myh7-as transcription may regulate the ratio of Myh6 and Myh7 (D). Some antisense transcripts are predicted to form RNA duplexes with their mRNA counterpart leading to post-transcriptional regulation of the target message. For example, antisense transcripts to Alc1 and cNTI, two genes that code for important sarcomere components in cardiac muscle, form RNA duplexes with the respective protein-coding transcript. Alc1 antisense is increased in hypertrophic ventricles in patients with Tetralogy of Fallot, whereas elevated cTNI levels are correlated with ischaemia and risk of heart failure. In both of these cases, antisense transcripts may be important for regulating gene expression through formation of RNA duplexes that are substrates for recruitment of factors that degrade the mRNA or that physically block translation of the message. RNA–RNA interactions can also stabilize the mRNA in some cases (E). Download figure Download PowerPoint While in vitro ESC-based cardiomyocyte differentiation recapitulates many of the stages of gastrulation and specification of the cardiac lineage (Kattman et al, 2007, 2011; Wamstad et al, 2012), it will also be critical to determine the function of Bvht during development on an organismal level. A conserved Bvht transcript was not identified in rat or human, suggesting that it is a rapidly evolving transcript (Klattenhoff et al, 2013). Although evidence of a transcript in rat and human is lacking, there is some DNA sequence conservation at syntenic sites among the three organisms making Bvht a particularly interesting example to study in terms of genomic evolution. Fendrr, an lncRNA necessary for heart and body wall development in mice The lncRNA Fendrr (Foxf1 adjacent non-coding developmental regulatory RNA; ENSMUSG00000097336) was recently identified in mouse as a potential regulator of heart development by virtue of its specific expression in the lateral plate mesoderm (Grote et al, 2013). The lateral plate mesoderm gives rise to the heart and structures of the ventral body wall. Loss of Fendrr resulted in embryonic lethality in mice (∼E13.5) and null embryos displayed open ventral body wall defects and hypoplastic ventricles, resulting in impaired heart function. Expression of a subset of cardiac transcription factors, Nkx2.5 and Gata6, was increased in Fendrr loss of function hearts accompanied by corresponding changes in H3K4me3 levels at their promoters, whereas other members of the core cardiac network, such as Gata4 and Tbx5, showed no changes. The Fendrr gene is proximal to Foxf1a, a gene that codes for a transcription factor involved in mesoderm formation. Consistent with a partial cis regulatory role for Fendrr, Foxf1a was also ectopically expressed in null animals. Fendrr interacted with PRC2 components as well as WDR5, a member of TrxG/MLL complex, to regulate mesoderm-specific genes. These results suggested that Fendrr regulates the balance between repressive and activating marks at key genes during development (Figure 3B), although it is not clear if both complexes simultaneously interact with the non-coding transcript. The authors suggest that targeting these complexes to specific genomic sites is in part mediated through interactions between predicted unstructured regions of Fendrr and DNA; however, this idea needs to be further experimentally tested. Interestingly, a syntenic transcript exists in the human genome (ENSG00000268388) suggesting a conserved role for Fendrr in human heart development. The interaction of Fendrr with multiple chromatin modifier complexes appears to be an emerging theme in lncRNA biology. For example, the lncRNA HOTAIR interacts with the PRC2 via its 5′ end and with the H3K4 demethylase LSD1 via its 3′ end, the Kcnq1ot1 lncRNA binds to both PRC2 and