Produção Nacional
Revisado por pares
Development of a method for extraction and assay of human erythrocyte acetylcholinesterase and pesticide inhibition
2013; SAGE Publishing; Volume: 32; Issue: 8 Linguagem: Inglês
10.1177/0960327112468906
ISSN1477-0903
AutoresAmanda Guedes Linhares, Caio Rodrigo Dias Assis, Marília Teixeira de Siqueira, Ranilson de Souza Bezerra, Luiz B. Carvalho,
Tópico(s)Pesticide and Herbicide Environmental Studies
ResumoA method of extracting membranes from red blood cells (RBCs) is described, which were in turn used to assay acetylcholinesterase (AChE) activity. The evidence for the enzyme activity was established by selective inhibition using 1,5- bis(4-allyldimethylammoniumphenyl) pentan-3-one dibromide, tetraisopropyl pyrophosphoramide and neostigmine. Blood samples were exposed to three organophosphorus (dichlorvos, chlorpyrifos and diazinon) and two carbamate (carbaryl and carbofuran) pesticides. Afterwards AChE activities in RBC membranes were determined. The concentrations capable to inhibit the enzyme activity by 50% (IC 50 ) for the pesticides were 10.66 µM (dichlorvos), 21.42 µM (chlorpyrifos), 109.98 µM (carbaryl) and 5.44 µM (carbofuran). The results related to 20% enzyme inhibition (level used in the estimation of threshold limits for anticholinesterase compounds) were below those acceptable daily intake values enacted by relevant national and international regulations. These results suggest that the proposed AChE extraction from RBC and assay could be a suitable method for monitoring occupational exposure to pesticides.
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