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MiR-361-5p inhibits colorectal and gastric cancer growth and metastasis by targeting staphylococcal nuclease domain containing-1

2015; Impact Journals LLC; Volume: 6; Issue: 19 Linguagem: Inglês

10.18632/oncotarget.3744

1949-2553

Fei Ma, Hongjiang Song, Baoliang Guo, Yuxin Zhang, Zheng Yasheng, Cheng-Chun Lin, Ying Wu, Guijie Guan, Ruihua Sha, Qingxin Zhou, Dejun Wang, Xinglu Zhou, Juan Li, Xiaohui Qiu,

Circular RNAs in diseases

// Fei Ma 1, * , Hongjiang Song 1, 2, * , Baoliang Guo 3, * , Yuxin Zhang 2 , Yasheng Zheng 4 , Chengchun Lin 5 , Ying Wu 6 , Guijie Guan 6 , Ruihua Sha 7 , Qingxin Zhou 3 , Dejun Wang 8 , Xinglu Zhou 9 , Juan Li 10 , Xiaohui Qiu 11 1 Department of Endoscopy, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China 2 Department of Gastrointestinal Surgery, The Affiliated Cancer Hospital, Harbin Medical University, Harbin, China 3 Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China 4 Department of General Surgery, Central Hospital of Jiuzhen, China 5 Department of Gastroenterology, The First Hospital of Longyan, Fujian Medical University, Longyan, China 6 Department of Pathology, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, China 7 Department of Digestive Disease, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, China 8 Department of Ultrasound of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China 9 Department of Medical Imaging, The Affiliated Cancer Hospital of Harbin Medical University, Harbin, China 10 Department of Pathology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China 11 The Second Hospital of Longyan, Fujian Medical University, Longyan, China * These authors have contributed equally to this work Correspondence to: Hongjiang Song, e-mail: hongjiangsong2015@163.com Keywords: miR-361-5p, Tudor staphylococcal nuclease 1, colorectal carcinoma, gastric cancer, RNA-induced silencing complex (RISC) Received: February 05, 2014 Accepted: April 06, 2015 Published: April 17, 2015 ABSTRACT MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, we investigated the biological role and mechanism of miR-361-5p in colorectal carcinoma (CRC) and gastric cancer (GC). We showed that microRNA-361-5p (miR-361-5p) was down-regulated in CRC and GC in comparison to the controls. Meanwhile, the expression levels of miR-361-5p negatively correlated with lung metastasis and prognosis in clinical CRC patients. Overexpression of miR-361-5p markedly suppressed proliferation, migration and invasion of cancer cells. Additionally, this phenotype could be partially rescued by the ectopic expression of staphylococcal nuclease domain containing-1 (SND1). SND1 was identified as a target of miR-361-5p using bioinformatics analysis and in vitro luciferase reporter assays. In turn, SND1 bound to pre-miR-361-5p and suppressed the expression of miR-361-5p, thus exerting a feedback loop. Most interestingly, in vivo studies showed that restoration of miR-361-5p significantly inhibited tumor growth and especially the lung metastasis in nude mice. Therefore, it could be concluded that miR-361-5p functions as a tumor-suppressive miRNA through directly binding to SND1, highlighting its potential as a novel agent for the treatment of patients with CRC and GC.